7p26
From Proteopedia
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==Sulfated host glycan recognition by carbohydrate sulfatases of the human gut microbiota (BT4631_S1_15)== | ==Sulfated host glycan recognition by carbohydrate sulfatases of the human gut microbiota (BT4631_S1_15)== | ||
| - | <StructureSection load='7p26' size='340' side='right'caption='[[7p26]]' scene=''> | + | <StructureSection load='7p26' size='340' side='right'caption='[[7p26]], [[Resolution|resolution]] 1.35Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P26 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P26 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7p26]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P26 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P26 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p26 OCA], [https://pdbe.org/7p26 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p26 RCSB], [https://www.ebi.ac.uk/pdbsum/7p26 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p26 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=P4K:polyethylene+glycol'>P4K</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p26 OCA], [https://pdbe.org/7p26 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p26 RCSB], [https://www.ebi.ac.uk/pdbsum/7p26 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p26 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sulfated glycans are ubiquitous nutrient sources for microbial communities that have coevolved with eukaryotic hosts. Bacteria metabolize sulfated glycans by deploying carbohydrate sulfatases that remove sulfate esters. Despite the biological importance of sulfatases, the mechanisms underlying their ability to recognize their glycan substrate remain poorly understood. Here, we use structural biology to determine how sulfatases from the human gut microbiota recognize sulfated glycans. We reveal seven new carbohydrate sulfatase structures spanning four S1 sulfatase subfamilies. Structures of S1_16 and S1_46 represent novel structures of these subfamilies. Structures of S1_11 and S1_15 demonstrate how non-conserved regions of the protein drive specificity toward related but distinct glycan targets. Collectively, these data reveal that carbohydrate sulfatases are highly selective for the glycan component of their substrate. These data provide new approaches for probing sulfated glycan metabolism while revealing the roles carbohydrate sulfatases play in host glycan catabolism. | ||
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| + | Sulfated glycan recognition by carbohydrate sulfatases of the human gut microbiota.,Luis AS, Basle A, Byrne DP, Wright GSA, London JA, Jin C, Karlsson NG, Hansson GC, Eyers PA, Czjzek M, Barbeyron T, Yates EA, Martens EC, Cartmell A Nat Chem Biol. 2022 Aug;18(8):841-849. doi: 10.1038/s41589-022-01039-x. Epub 2022, Jun 16. PMID:35710619<ref>PMID:35710619</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7p26" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cartmell, A]] | [[Category: Cartmell, A]] | ||
| + | [[Category: Carbohydrate sulfatase]] | ||
| + | [[Category: Host glycan]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Microbiota]] | ||
| + | [[Category: Sulfation]] | ||
Revision as of 05:08, 10 August 2022
Sulfated host glycan recognition by carbohydrate sulfatases of the human gut microbiota (BT4631_S1_15)
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