3siu

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<StructureSection load='3siu' size='340' side='right'caption='[[3siu]], [[Resolution|resolution]] 2.63&Aring;' scene=''>
<StructureSection load='3siu' size='340' side='right'caption='[[3siu]], [[Resolution|resolution]] 2.63&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3siu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SIU FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3siu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SIU FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.626&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ozb|2ozb]], [[3siv|3siv]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NHP2L1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), PRP31, PRPF31 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3siu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3siu OCA], [https://pdbe.org/3siu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3siu RCSB], [https://www.ebi.ac.uk/pdbsum/3siu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3siu ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3siu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3siu OCA], [https://pdbe.org/3siu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3siu RCSB], [https://www.ebi.ac.uk/pdbsum/3siu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3siu ProSAT]</span></td></tr>
</table>
</table>
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== Disease ==
 
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[[https://www.uniprot.org/uniprot/PRP31_HUMAN PRP31_HUMAN]] Defects in PRPF31 are the cause of retinitis pigmentosa type 11 (RP11) [MIM:[https://omim.org/entry/600138 600138]]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP11 inheritance is autosomal dominant.<ref>PMID:17412961</ref> <ref>PMID:12444105</ref> <ref>PMID:11545739</ref> <ref>PMID:8808602</ref> <ref>PMID:12923864</ref>
 
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/NH2L1_HUMAN NH2L1_HUMAN]] Binds to the 5'-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding.<ref>PMID:10545122</ref> <ref>PMID:17412961</ref> [[https://www.uniprot.org/uniprot/PRP31_HUMAN PRP31_HUMAN]] Involved in pre-mRNA splicing. Required for U4/U6.U5 tri-snRNP formation.<ref>PMID:11867543</ref>
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[https://www.uniprot.org/uniprot/NH2L1_HUMAN NH2L1_HUMAN] Binds to the 5'-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly. The protein undergoes a conformational change upon RNA-binding.<ref>PMID:10545122</ref> <ref>PMID:17412961</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human proteins 15.5K and hPrp31 are components of the major spliceosomal U4 snRNP and of the minor spliceosomal U4atac snRNP. The two proteins bind to related 5'-stem loops (5'SLs) of the U4 and U4atac snRNAs in a strictly sequential fashion. The primary binding 15.5K protein binds at K-turns that exhibit identical sequences in the two snRNAs. However, RNA sequences contacted by the secondary binding hPrp31 differ in U4 and U4atac snRNAs, and the mechanism by which hPrp31 achieves its dual specificity is presently unknown. We show by crystal structure analysis that the capping pentaloops of the U4 and U4atac 5'SLs adopt different structures in the ternary hPrp31-15.5K-snRNA complexes. In U4atac snRNA, a noncanonical base pair forms across the pentaloop, based on which the RNA establishes more intimate interactions with hPrp31 compared with U4 snRNA. Stacking of hPrp31-His270 on the noncanonical base pair at the base of the U4atac pentaloop recapitulates intramolecular stabilizing principles known from the UUCG and GNRA families of RNA tetraloops. Rational mutagenesis corroborated the importance of the noncanonical base pair and the U4atac-specific hPrp31-RNA interactions for complex stability. The more extensive hPrp31-U4atac snRNA interactions are in line with a higher stability of the U4atac compared with the U4-based ternary complex seen in gel-shift assays, which may explain how U4atac snRNA can compete with the more abundant U4 snRNA for the same protein partners in vivo.
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Structural basis for the dual U4 and U4atac snRNA-binding specificity of spliceosomal protein hPrp31.,Liu S, Ghalei H, Luhrmann R, Wahl MC RNA. 2011 Jul 22. PMID:21784869<ref>PMID:21784869</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3siu" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
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[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Ghalei, H]]
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[[Category: Ghalei H]]
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[[Category: Liu, S]]
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[[Category: Liu S]]
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[[Category: Luhrmann, R]]
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[[Category: Luhrmann R]]
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[[Category: Wahl, M C]]
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[[Category: Wahl MC]]
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[[Category: Major and minor spliceosome]]
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[[Category: Nucleus]]
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[[Category: Pre-mrna splicing]]
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[[Category: Rna-binding protein]]
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[[Category: Rna-protein complex]]
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[[Category: Splicing-rna complex]]
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[[Category: U4 snrna]]
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[[Category: U4 snrnp and u4atac snrnp]]
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Current revision

Structure of a hPrp31-15.5K-U4atac 5' stem loop complex, monomeric form

PDB ID 3siu

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