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1hl7

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[[Image:1hl7.gif|left|200px]]
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{{STRUCTURE_1hl7| PDB=1hl7 | SCENE= }}
{{STRUCTURE_1hl7| PDB=1hl7 | SCENE= }}
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'''GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES IN COMPLEX WITH 3A,4,7,7A-TETRAHYDRO-BENZO [1,3] DIOXOL-2-ONE'''
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===GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES IN COMPLEX WITH 3A,4,7,7A-TETRAHYDRO-BENZO [1,3] DIOXOL-2-ONE===
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==Overview==
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The structure of the recombinant (-) gamma-lactamase from an Aureobacterium species has been solved at 1.73A resolution in the cubic space group F23 with unit cell parameters a=b=c=240.6A. The trimeric enzyme has an alpha/beta hydrolase fold and closely resembles the cofactor free haloperoxidases. The structure has been solved in complex with a covalently bound ligand originating from the host cell and also in the unligated form. The associated density in the former structure has been interpreted as the two-ring ligand (3aR,7aS)-3a,4,7,7a-tetrahydro-benzo [1,3] dioxol-2-one which forms a tetrahedral complex with OG of the catalytic Ser98. Soaks of these crystals with the industrial substrate gamma-lactam or its structural analogue, norcamphor, result in the displacement of the ligand from the enzyme active site, thereby allowing determination of the unligated structure. The presence of the ligand in the active site protects the enzyme from serine hydrolase inhibitors. Cyclic ethylene carbonate, the first ring of the ligand, was shown to be a substrate of the enzyme.
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(as it appears on PubMed at http://www.pubmed.gov), where 15081810 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15081810}}
==About this Structure==
==About this Structure==
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[[Category: Co-factor free haloperoxidase]]
[[Category: Co-factor free haloperoxidase]]
[[Category: Tetrahedral intermediate]]
[[Category: Tetrahedral intermediate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:58:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:20:48 2008''

Revision as of 05:20, 1 July 2008

Image:1hl7.png

Template:STRUCTURE 1hl7

GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES IN COMPLEX WITH 3A,4,7,7A-TETRAHYDRO-BENZO [1,3] DIOXOL-2-ONE

Template:ABSTRACT PUBMED 15081810

About this Structure

1HL7 is a Single protein structure of sequence from Microbacterium. Full crystallographic information is available from OCA.

Reference

The crystal structure of a (-) gamma-lactamase from an Aureobacterium species reveals a tetrahedral intermediate in the active site., Line K, Isupov MN, Littlechild JA, J Mol Biol. 2004 Apr 30;338(3):519-32. PMID:15081810

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