1fli
From Proteopedia
(New page: 200px<br /> <applet load="1fli" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fli" /> '''DNA-BINDING DOMAIN OF FLI-1'''<br /> ==Ove...) |
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'''DNA-BINDING DOMAIN OF FLI-1'''<br /> | '''DNA-BINDING DOMAIN OF FLI-1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Members of the ets family of transcription factors share a conserved | + | Members of the ets family of transcription factors share a conserved DNA-binding domain, the ets domain. By using multidimensional NMR, we have determined the structure of the ets domain of human Fli-1 in the DNA-bound form. It consists of three alpha-helices and a four-stranded beta-sheet, similar to structures of the class of helix-turn-helix DNA binding proteins first found in the catabolite activator protein of Escherichia coli. NMR and mutagenesis experiments suggest that in comparison to structurally related proteins, the ets domain uses a new variation of the helix-turn-helix motif for binding to DNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1FLI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1FLI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FLI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Fesik, S | + | [[Category: Fesik, S W.]] |
[[Category: Liang, H.]] | [[Category: Liang, H.]] | ||
[[Category: Mao, X.]] | [[Category: Mao, X.]] | ||
| - | [[Category: Meadows, R | + | [[Category: Meadows, R P.]] |
| - | [[Category: Nettesheim, D | + | [[Category: Nettesheim, D G.]] |
| - | [[Category: Olejniczak, E | + | [[Category: Olejniczak, E T.]] |
| - | [[Category: Thompson, C | + | [[Category: Thompson, C B.]] |
[[Category: Yu, L.]] | [[Category: Yu, L.]] | ||
[[Category: transcription/dna]] | [[Category: transcription/dna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:39:53 2008'' |
Revision as of 10:39, 21 February 2008
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DNA-BINDING DOMAIN OF FLI-1
Overview
Members of the ets family of transcription factors share a conserved DNA-binding domain, the ets domain. By using multidimensional NMR, we have determined the structure of the ets domain of human Fli-1 in the DNA-bound form. It consists of three alpha-helices and a four-stranded beta-sheet, similar to structures of the class of helix-turn-helix DNA binding proteins first found in the catabolite activator protein of Escherichia coli. NMR and mutagenesis experiments suggest that in comparison to structurally related proteins, the ets domain uses a new variation of the helix-turn-helix motif for binding to DNA.
About this Structure
1FLI is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of the ets domain of Fli-1 when bound to DNA., Liang H, Mao X, Olejniczak ET, Nettesheim DG, Yu L, Meadows RP, Thompson CB, Fesik SW, Nat Struct Biol. 1994 Dec;1(12):871-5. PMID:7773776
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