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6v99

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<StructureSection load='6v99' size='340' side='right'caption='[[6v99]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
<StructureSection load='6v99' size='340' side='right'caption='[[6v99]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6v99]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrfc Agrfc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6V99 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6v99]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_fabrum_str._C58 Agrobacterium fabrum str. C58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6V99 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">glgC, Atu4076, AGR_L_1560 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=176299 AGRFC])</td></tr>
 
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glucose-1-phosphate_adenylyltransferase Glucose-1-phosphate adenylyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.27 2.7.7.27] </span></td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6v99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v99 OCA], [https://pdbe.org/6v99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6v99 RCSB], [https://www.ebi.ac.uk/pdbsum/6v99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6v99 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6v99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v99 OCA], [https://pdbe.org/6v99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6v99 RCSB], [https://www.ebi.ac.uk/pdbsum/6v99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6v99 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/GLGC_AGRFC GLGC_AGRFC]] Involved in the biosynthesis of ADP-glucose, a building block required for the elongation reactions to produce glycogen. Catalyzes the reaction between ATP and alpha-D-glucose 1-phosphate (G1P) to produce pyrophosphate and ADP-Glc.[HAMAP-Rule:MF_00624]
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[[https://www.uniprot.org/uniprot/GLGC_AGRFC GLGC_AGRFC]] Involved in the biosynthesis of ADP-glucose, a building block required for the elongation reactions to produce glycogen. Catalyzes the reaction between ATP and alpha-D-glucose 1-phosphate (G1P) to produce pyrophosphate and ADP-Glc.[HAMAP-Rule:MF_00624]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The allosteric regulation of ADP-glucose pyrophosphorylase is critical for the biosynthesis of glycogen in bacteria and starch in plants. The enzyme from Agrobacterium tumefaciens is activated by fructose 6-phosphate (Fru6P) and pyruvate (Pyr). The Pyr site has been recently found, but the site where Fru6P binds has remained unknown. We hypothesize that a sulfate ion previously found in the crystal structure reveals a part of the regulatory site mimicking the presence of the phosphoryl moiety of the activator Fru6P. Ser72 interacts with this sulfate ion and, if the hypothesis is correct, Ser72 would affect the interaction with Fru6P and activation of the enzyme. Here, we report structural, binding, and kinetic analysis of Ser72 mutants of the A. tumefaciens ADP-glucose pyrophosphorylase. By X-ray crystallography, we found that when Ser72 was replaced by Asp or Glu side chain carboxylates protruded into the sulfate-binding pocket. They would present a strong steric and electrostatic hindrance to the phosphoryl moiety of Fru6P, while being remote from the Pyr site. In agreement, we found that Fru6P could not activate or bind to S72E or S72D mutants, whereas Pyr was still an effective activator. These mutants also blocked the binding of the inhibitor AMP. This could potentially have biotechnological importance in obtaining enzyme forms insensitive to inhibition. Other mutations in this position (Ala, Cys, and Trp) confirmed the importance of Ser72 in regulation. We propose that the ADP-glucose pyrophosphorylase from A. tumefaciens have two distinct sites for Fru6P and Pyr working in tandem to regulate glycogen biosynthesis.
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Site-directed mutagenesis of Serine-72 reveals the location of the fructose 6-phosphate regulatory site of the Agrobacterium tumefaciens ADP-glucose pyrophosphorylase.,Alghamdi MA, Hussien RA, Zheng Y, Patel HP, Asencion Diez MD, A Iglesias A, Liu D, Ballicora MA Protein Sci. 2022 Jul;31(7):e4376. doi: 10.1002/pro.4376. PMID:35762722<ref>PMID:35762722</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6v99" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Agrfc]]
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[[Category: Agrobacterium fabrum str. C58]]
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[[Category: Glucose-1-phosphate adenylyltransferase]]
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[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Alghamdi, M A]]
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[[Category: Alghamdi MA]]
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[[Category: Ballicora, M A]]
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[[Category: Ballicora MA]]
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[[Category: Liu, D]]
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[[Category: Liu D]]
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[[Category: Zheng, Y]]
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[[Category: Zheng Y]]
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[[Category: Allosteric regulation]]
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[[Category: Enzyme structure]]
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[[Category: Fructose 6-phosphate]]
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[[Category: Glycogen synthesis]]
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[[Category: Transferase]]
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Revision as of 07:08, 8 September 2022

Agrobacterium tumefaciens ADP-Glucose pyrophosphorylase- S72D in the presence of sulfate

PDB ID 6v99

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