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3t12

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<StructureSection load='3t12' size='340' side='right'caption='[[3t12]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='3t12' size='340' side='right'caption='[[3t12]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3t12]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T12 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T12 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3t12]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T12 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T12 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3t1o|3t1o]], [[3t1q|3t1q]], [[3t1r|3t1r]], [[3t1s|3t1s]], [[3t1t|3t1t]], [[3t1v|3t1v]], [[3t1x|3t1x]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TTHA1132 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8]), TTHA1131 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t12 OCA], [https://pdbe.org/3t12 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t12 RCSB], [https://www.ebi.ac.uk/pdbsum/3t12 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t12 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t12 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t12 OCA], [https://pdbe.org/3t12 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t12 RCSB], [https://www.ebi.ac.uk/pdbsum/3t12 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t12 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q5SJ82_THET8 Q5SJ82_THET8]
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The bacterium Myxococcus xanthus uses a G protein cycle to dynamically regulate the leading/lagging pole polarity axis. The G protein MglA is regulated by its GTPase-activating protein (GAP) MglB, thus resembling Ras family proteins. Here, we show structurally and biochemically that MglA undergoes a dramatic, GDP-GTP-dependent conformational change involving a screw-type forward movement of the central beta2-strand, never observed in any other G protein. This movement and complex formation with MglB repositions the conserved residues Arg53 and Gln82 into the active site. Residues required for catalysis are thus not provided by the GAP MglB, but by MglA itself. MglB is a Roadblock/LC7 protein and functions as a dimer to stimulate GTP hydrolysis in a 2:1 complex with MglA. In vivo analyses demonstrate that hydrolysis mutants abrogate Myxococcus' ability to regulate its polarity axis changing the reversal behaviour from stochastic to oscillatory and that both MglA GTPase activity and MglB GAP catalysis are essential for maintaining a proper polarity axis.
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Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity.,Miertzschke M, Koerner C, Vetter IR, Keilberg D, Hot E, Leonardy S, Sogaard-Andersen L, Wittinghofer A EMBO J. 2011 Aug 16. doi: 10.1038/emboj.2011.291. PMID:21847100<ref>PMID:21847100</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3t12" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Gliding protein|Gliding protein]]
*[[Gliding protein|Gliding protein]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Small monomeric GTPase]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Thet8]]
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[[Category: Koerner C]]
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[[Category: Koerner, C]]
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[[Category: Miertzschke M]]
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[[Category: Miertzschke, M]]
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[[Category: Vetter IR]]
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[[Category: Vetter, I R]]
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[[Category: Wittinghofer A]]
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[[Category: Wittinghofer, A]]
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[[Category: Alpha/beta protein]]
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[[Category: Bacterial polarity]]
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[[Category: G-domain containing protein]]
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[[Category: Gtpase]]
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[[Category: Homodimeric gap protein]]
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[[Category: Hydrolase-signaling protein complex]]
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[[Category: Motility]]
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[[Category: Pole localisation]]
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Current revision

MglA in complex with MglB in transition state

PDB ID 3t12

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