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| | <StructureSection load='3t8j' size='340' side='right'caption='[[3t8j]], [[Resolution|resolution]] 1.60Å' scene=''> | | <StructureSection load='3t8j' size='340' side='right'caption='[[3t8j]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3t8j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T8J FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3t8j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T8J FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2mas|2mas]], [[1q8f|1q8f]], [[3g5i|3g5i]]</div></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">iunH-1, SSO0505 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr> | + | |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Purine_nucleosidase Purine nucleosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.1 3.2.2.1] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t8j OCA], [https://pdbe.org/3t8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t8j RCSB], [https://www.ebi.ac.uk/pdbsum/3t8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t8j ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t8j OCA], [https://pdbe.org/3t8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t8j RCSB], [https://www.ebi.ac.uk/pdbsum/3t8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t8j ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q97ZS5_SACS2 Q97ZS5_SACS2] |
| - | The purine and pyrimidine-specific nucleoside hydrolases (NHs) from the archaeon Sulfolobus solfataricus participate in the fundamental pathway of nucleotide catabolism, and concur in maintaining adequate levels of free nitrogenous bases for cellular function. The two highly homologous isozymes display distinct specificities towards nucleoside substrates, and both lack the amino acids employed for activation of the leaving group in the hydrolytic reaction by the so far characterized NHs. We determined the high-resolution crystal structures of the purine and pyrimidine-specific NHs from S. solfataricus to reveal that both enzymes belong to the NH structural homology group I, despite the different substrate specificities. A Na+ ion is bound at the active site of the pyrimidine-specific NH instead of the prototypical Ca2+, delineating a role of the metals in the catalytic mechanism of NHs in the substrate binding rather than nucleophile activation. A conserved His residue, that regulates product release in other homologous NHs, provides crucial interactions for leaving group activation in the archaeal isozymes. Modeling of the enzyme-substrate interactions suggests that steric exclusion and catalytic selection underlie the orthogonal base specificity of the two isozymes.
| + | |
| - | | + | |
| - | New determinants in the catalytic mechanism of nucleoside hydrolases from the structures of two isozymes from Sulfolobus solfataricus.,Minici C, Cacciapuoti G, De Leo E, Porcelli M, Degano M Biochemistry. 2012 May 2. PMID:22551416<ref>PMID:22551416</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3t8j" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Purine nucleosidase]] | + | [[Category: Saccharolobus solfataricus P2]] |
| - | [[Category: Sacs2]] | + | [[Category: Cacciapuoti G]] |
| - | [[Category: Cacciapuoti, G]] | + | [[Category: De Leo E]] |
| - | [[Category: Degano, M]] | + | [[Category: Degano M]] |
| - | [[Category: Leo, E De]]
| + | [[Category: Minici C]] |
| - | [[Category: Minici, C]] | + | [[Category: Porcelli M]] |
| - | [[Category: Porcelli, M]] | + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: N-glycosidase]]
| + | |
| - | [[Category: Nh-fold]]
| + | |
| - | [[Category: Nucleoside hydrolase]]
| + | |
| - | [[Category: Nucleotide metabolism]]
| + | |
| - | [[Category: Rossmann fold]]
| + | |
| - | [[Category: Thermostable protein]]
| + | |
