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3tix

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<StructureSection load='3tix' size='340' side='right'caption='[[3tix]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
<StructureSection load='3tix' size='340' side='right'caption='[[3tix]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3tix]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TIX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3tix]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C] and [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TIX FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9001&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tas3, SPBC83.03c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), chp1, SPAC18G6.02c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tix OCA], [https://pdbe.org/3tix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tix RCSB], [https://www.ebi.ac.uk/pdbsum/3tix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tix ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tix OCA], [https://pdbe.org/3tix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tix RCSB], [https://www.ebi.ac.uk/pdbsum/3tix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tix ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/SMT3_YEAST SMT3_YEAST]] Not known; suppressor of MIF2 mutations. [[https://www.uniprot.org/uniprot/CHP1_SCHPO CHP1_SCHPO]] Component of the kinetochore which plays a role in stabilizing microtubules and so allowing accurate chromosome segregation. Has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation and accurate chromosome segregation. A member of the RNA-induced transcriptional silencing (RITS) complex which is involved in the biosynthesis of dsRNA from primer siRNAs provided by the RNA-directed RNA polymerase (RDRC) complex.<ref>PMID:9722643</ref> <ref>PMID:10835380</ref> <ref>PMID:15607976</ref> <ref>PMID:14704433</ref>
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[https://www.uniprot.org/uniprot/TAS3_SCHPO TAS3_SCHPO] Has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation and accurate chromosome segregation. A member of the RNA-induced transcriptional silencing (RITS) complex which is involved in the biosynthesis of dsRNA from primer siRNAs provided by the RNA-directed RNA polymerase (RDRC) complex.<ref>PMID:15607976</ref> <ref>PMID:14704433</ref> [https://www.uniprot.org/uniprot/SMT3_YEAST SMT3_YEAST] Not known; suppressor of MIF2 mutations.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RNA interference (RNAi) is critical for the assembly of heterochromatin at Schizosaccharomyces pombe centromeres. Central to this process is the RNA-induced initiation of transcriptional gene silencing (RITS) complex, which physically anchors small noncoding RNAs to chromatin. RITS includes Ago1, the chromodomain protein Chp1, and Tas3, which forms a bridge between Chp1 and Ago1. Chp1 is a large protein with no recognizable domains, apart from its chromodomain. Here we describe how the structured C-terminal half of Chp1 binds the Tas3 N-terminal domain, revealing the tight association of Chp1 and Tas3. The structure also shows a PIN domain at the C-terminal tip of Chp1 that controls subtelomeric transcripts through a post-transcriptional mechanism. We suggest that the Chp1-Tas3 complex provides a solid and versatile platform to recruit both RNAi-dependent and RNAi-independent gene-silencing pathways for locus-specific regulation of heterochromatin.
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The Chp1-Tas3 core is a multifunctional platform critical for gene silencing by RITS.,Schalch T, Job G, Shanker S, Partridge JF, Joshua-Tor L Nat Struct Mol Biol. 2011 Nov 13;18(12):1351-7. doi: 10.1038/nsmb.2151. PMID:22081013<ref>PMID:22081013</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3tix" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Fission yeast]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Joshua-Tor, L]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Schalch, T]]
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[[Category: Schizosaccharomyces pombe 972h-]]
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[[Category: Alpha-helical hairpin]]
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[[Category: Joshua-Tor L]]
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[[Category: Argonaute]]
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[[Category: Schalch T]]
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[[Category: Clrc]]
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[[Category: Gene regulation-protein binding complex]]
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[[Category: Heterochromatin]]
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[[Category: Nucleus]]
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[[Category: Pin]]
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[[Category: Rdrc]]
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[[Category: Rit]]
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[[Category: Rnai]]
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[[Category: Rossmann fold]]
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[[Category: Silencing]]
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[[Category: Spoc]]
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Current revision

Crystal structure of the Chp1-Tas3 complex core

PDB ID 3tix

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