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7y8p

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'''Unreleased structure'''
 
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The entry 7y8p is ON HOLD until Paper Publication
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==Crystal structure of 4'-selenoRNA duplex==
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<StructureSection load='7y8p' size='340' side='right'caption='[[7y8p]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[7y8p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Y8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Y8P FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IKS:[(2~{R},3~{S},4~{R},5~{R})-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)selenolan-2-yl]methyl+dihydrogen+phosphate'>IKS</scene>, <scene name='pdbligand=ILK:[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)selenolan-2-yl]methyl+dihydrogen+phosphate'>ILK</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7y8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7y8p OCA], [https://pdbe.org/7y8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7y8p RCSB], [https://www.ebi.ac.uk/pdbsum/7y8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7y8p ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A large number of chemically modified oligonucleotides (ONs) have been developed for RNA-based technologies. In most modified RNAs, the characteristic 2'-hydroxyl (2'-OH) groups are removed to enhance both nuclease resistance and hybridization ability. However, the importance of the 2'-OH group in RNA structure and function is well known. Here, we report the synthesis and properties of 4'-selenoRNA in which all four nucleoside units retain the 2'-OH groups but contain a selenium atom instead of an oxygen atom at the 4'-position of the furanose ring. 4'-SelenoRNA has enhanced ability to form duplexes with RNA, and high endonuclease resistance despite the presence of the 2'-OH groups. X-ray crystallography analysis showed that the 4'-selenoRNA duplex adopts an A-conformation, similar to natural RNA, although one 4'-selenocytidine residue has unusual South-type sugar puckering. Furthermore, preliminary studies using 4'-seleno-modified siRNAs suggest that 4'-selenoRNA may be applicable to RNA interference technology. Collectively, our results raise the possibility of a new class of modified RNA in which 2'-OH groups do not need to be masked.
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Authors: Kondo, J., Minakawa, N., Ohta, M., Takahashi, H., Tarashima, N.
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Synthesis and properties of fully-modified 4'-selenoRNA, an endonuclease-resistant RNA analog.,Ota M, Takahashi H, Nogi Y, Kagotani Y, Saito-Tarashima N, Kondo J, Minakawa N Bioorg Med Chem. 2022 Dec 15;76:117093. doi: 10.1016/j.bmc.2022.117093. Epub 2022 , Nov 19. PMID:36434923<ref>PMID:36434923</ref>
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Description: Crystal structure of 4''-selenoRNA duplex
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Ohta, M]]
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<div class="pdbe-citations 7y8p" style="background-color:#fffaf0;"></div>
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[[Category: Kondo, J]]
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== References ==
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[[Category: Takahashi, H]]
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<references/>
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[[Category: Tarashima, N]]
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__TOC__
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[[Category: Minakawa, N]]
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
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[[Category: Kondo J]]
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[[Category: Minakawa N]]
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[[Category: Ohta M]]
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[[Category: Takahashi H]]
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[[Category: Tarashima N]]

Revision as of 07:26, 3 May 2023

Crystal structure of 4'-selenoRNA duplex

PDB ID 7y8p

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OCA

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