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8a1h
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Bacterial 6-4 photolyase from Vibrio cholerase== | |
| + | <StructureSection load='8a1h' size='340' side='right'caption='[[8a1h]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8a1h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A1H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A1H FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DLZ:1-DEOXY-1-(6,7-DIMETHYL-2,4-DIOXO-3,4-DIHYDROPTERIDIN-8(2H)-YL)-D-RIBITOL'>DLZ</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a1h OCA], [https://pdbe.org/8a1h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a1h RCSB], [https://www.ebi.ac.uk/pdbsum/8a1h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a1h ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9KLD7_VIBCH Q9KLD7_VIBCH] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Photolyases are flavoproteins, which are able to repair UV-induced DNA lesions in a light-dependant manner. According to their substrate, they can be distinguished as CPD- and (6-4) photolyases. While CPD-photolyases repair the predominantly occurring cyclobutane pyrimidine dimer lesion, (6-4) photolyases catalyse the repair of the less prominent (6-4) photoproduct. The subgroup of prokaryotic (6-4) photolyases/FeS-BCP is one of the most ancient types of flavoproteins in the ubiquitously occurring photolyase & cryptochrome superfamily (PCSf). In contrast to canonical photolyases, prokaryotic (6-4) photolyases possess a few particular characteristics, including a lumazine derivative as antenna chromophore beside the catalytically essential flavin adenine dinucleotide as well as an elongated linker region between the N-terminal alpha/beta-domain and the C-terminal all-alpha-helical domain. Furthermore, they can harbour an additional short subdomain, located at the C-terminus, with a binding site for a [4Fe-4S] cluster. So far two crystal structures of prokaryotic (6-4) photolyases have been reported. Within this study we present the high-resolution structure of the prokaryotic (6-4) photolyase from Vibrio cholerae and its spectroscopic characterization in terms of in vitro photoreduction and DNA-repair activity. | ||
| - | + | Structural and Functional Analysis of a Prokaryotic (6-4) Photolyase from the Aquatic Pathogen Vibrio Cholerae.,Emmerich HJ, Schneider L, Essen LO Photochem Photobiol. 2023 Jan 24. doi: 10.1111/php.13783. PMID:36692077<ref>PMID:36692077</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Emmerich | + | <div class="pdbe-citations 8a1h" style="background-color:#fffaf0;"></div> |
| - | [[Category: Essen | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Vibrio cholerae]] | ||
| + | [[Category: Emmerich HJ]] | ||
| + | [[Category: Essen L-O]] | ||
Revision as of 06:45, 8 February 2023
Bacterial 6-4 photolyase from Vibrio cholerase
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