8acb
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==CryoEM structure of sweet potato feathery mottle virus VLP== | |
| + | <StructureSection load='8acb' size='340' side='right'caption='[[8acb]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8acb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ipomoea_batatas Ipomoea batatas] and [https://en.wikipedia.org/wiki/Sweet_potato_feathery_mottle_virus Sweet potato feathery mottle virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ACB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ACB FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8acb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8acb OCA], [https://pdbe.org/8acb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8acb RCSB], [https://www.ebi.ac.uk/pdbsum/8acb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8acb ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/D2CTM1_9POTV D2CTM1_9POTV] Involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.[ARBA:ARBA00029405] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sweet potato feathery mottle virus (SPFMV) and Sweet potato mild mottle virus (SPMMV) are members of the genera Potyvirus and Ipomovirus, family Potyviridae, sharing Ipomoea batatas as common host, but transmitted, respectively, by aphids and whiteflies. Virions of family members consist of flexuous rods with multiple copies of a single coat protein (CP) surrounding the RNA genome. Here we report the generation of virus-like particles (VLPs) by transient expression of the CPs of SPFMV and SPMMV in the presence of a replicating RNA in Nicotiana benthamiana. Analysis of the purified VLPs by cryo-electron microscopy, gave structures with resolutions of 2.6 and 3.0 A, respectively, showing a similar left-handed helical arrangement of 8.8 CP subunits per turn with the C-terminus at the inner surface and a binding pocket for the encapsidated ssRNA. Despite their similar architecture, thermal stability studies reveal that SPMMV VLPs are more stable than those of SPFMV. | ||
| - | + | CryoEM and stability analysis of virus-like particles of potyvirus and ipomovirus infecting a common host.,Chase O, Javed A, Byrne MJ, Thuenemann EC, Lomonossoff GP, Ranson NA, Lopez-Moya JJ Commun Biol. 2023 Apr 19;6(1):433. doi: 10.1038/s42003-023-04799-x. PMID:37076658<ref>PMID:37076658</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8acb" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Byrne | + | <references/> |
| - | [[Category: Lomonosoff | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Ipomoea batatas]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Sweet potato feathery mottle virus]] | ||
| + | [[Category: Byrne JM]] | ||
| + | [[Category: Javed A]] | ||
| + | [[Category: Lomonosoff G]] | ||
| + | [[Category: Ranson N]] | ||
Revision as of 06:58, 18 May 2023
CryoEM structure of sweet potato feathery mottle virus VLP
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