8d8k
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Yeast mitochondrial small subunit assembly intermediate (State 2)== | |
| + | <StructureSection load='8d8k' size='340' side='right'caption='[[8d8k]], [[Resolution|resolution]] 3.13Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8d8k]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8D8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8D8K FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8d8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8d8k OCA], [https://pdbe.org/8d8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8d8k RCSB], [https://www.ebi.ac.uk/pdbsum/8d8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8d8k ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RT22_YEAST RT22_YEAST] Probable S-adenosyl-L-methionine-dependent RNA methyltransferase (PubMed:19351663, PubMed:24651469). May provide the activity for methylation of the rRNA of the small mitochondrial subunit, which is required for the assembly and stability of the mitochondrial ribosome (PubMed:22689777). Has no protein methyltransferase activity (PubMed:24651469).<ref>PMID:24651469</ref> <ref>PMID:19351663</ref> <ref>PMID:22689777</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is essential for ATP production and cellular metabolism(1). Here we used cryo-electron microscopy to determine 9 structures of native yeast and human mitoribosomal small subunit assembly intermediates at resolutions from 2.4 to 3.8 A, illuminating the mechanistic basis for how GTPases are employed to control early steps of decoding center formation, how initial rRNA folding and processing events are mediated, and how mitoribosomal proteins play active roles during assembly. Furthermore, this series of intermediates from two species with divergent mitoribosomal architecture uncovers both conserved principles and species-specific adaptations that govern the maturation of mitoribosomal small subunits in eukaryotes. By revealing the dynamic interplay between assembly factors, mitoribosomal proteins, and rRNA required to generate functional subunits, our structural analysis provides a vignette for how molecular complexity and diversity can evolve in large ribonucleoprotein assemblies. | ||
| - | + | Principles of mitoribosomal small subunit assembly in eukaryotes.,Harper NJ, Burnside C, Klinge S Nature. 2022 Dec 8. doi: 10.1038/s41586-022-05621-0. PMID:36482135<ref>PMID:36482135</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8d8k" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae]] | ||
| + | [[Category: Burnside C]] | ||
| + | [[Category: Harper NJ]] | ||
| + | [[Category: Klinge S]] | ||
Current revision
Yeast mitochondrial small subunit assembly intermediate (State 2)
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