7wcj

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<StructureSection load='7wcj' size='340' side='right'caption='[[7wcj]], [[Resolution|resolution]] 2.24&Aring;' scene=''>
<StructureSection load='7wcj' size='340' side='right'caption='[[7wcj]], [[Resolution|resolution]] 2.24&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7wcj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WCJ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7wcj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WCJ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7wda|7wda]]</div></td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wcj OCA], [https://pdbe.org/7wcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wcj RCSB], [https://www.ebi.ac.uk/pdbsum/7wcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wcj ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wcj OCA], [https://pdbe.org/7wcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wcj RCSB], [https://www.ebi.ac.uk/pdbsum/7wcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wcj ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/LPQY_MYCTU LPQY_MYCTU]] Part of the ABC transporter complex LpqY-SugA-SugB-SugC, which is highly specific for uptake of trehalose. Involved in the recycling of extracellular trehalose released from trehalose-containing molecules synthesized by M.tuberculosis. Trehalose uptake is essential for virulence.<ref>PMID:21118978</ref>
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[[https://www.uniprot.org/uniprot/LPQY_MYCTU LPQY_MYCTU]] Part of the ABC transporter complex LpqY-SugA-SugB-SugC, which is highly specific for uptake of trehalose. Involved in the recycling of extracellular trehalose released from trehalose-containing molecules synthesized by M.tuberculosis. Trehalose uptake is essential for virulence.<ref>PMID:21118978</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The LpqY-SugABC transporter of Mycobacterium tuberculosis (Mtb) salvages residual trehalose across the cell membrane, which is otherwise lost during the formation of cell-wall glycoconjugates in the periplasm. LpqY, a substrate-binding protein from the SugABC transporter, acts as the primary receptor for the recognition of trehalose, leading to its transport across the cell membrane. Since trehalose is crucial for the survival and virulence of Mtb, trehalose receptors should serve as important targets for novel drug design against tuberculosis. In order to comprehend the detailed architecture and substrate specificity, the first crystal structures of both apo and trehalose-bound forms of M. tuberculosis LpqY (Mtb-LpqY) are presented here at 2.2 and 1.9 A resolution, respectively. The structure exhibits an N-lobe and C-lobe and is predominantly composed of a globular alpha/beta domain connected by a flexible hinge region concealing a deep binding cleft. Although the trehalose-bound form of Mtb-LpqY revealed an open ligand-bound conformation, the glucose moieties of trehalose are seen to be strongly held in place by direct and water-mediated hydrogen bonds within the binding cavity, producing a Kd of 6.58 +/- 1.21 microM. These interactions produce a distinct effect on the stereoselectivity for the alpha-1,1-glycosidic linkage of trehalose. Consistent with the crystal structure, molecular-dynamics simulations further validated Asp43, Asp97 and Asn151 as key residues responsible for strong and stable interactions throughout a 1 micros time frame, thus capturing trehalose in the binding cavity. Collectively, the results provide detailed insights into how the structure and dynamics of Mtb-LpqY enable it to specifically bind trehalose in a relaxed conformation state.
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Structural analysis of LpqY, a substrate-binding protein from the SugABC transporter of Mycobacterium tuberculosis, provides insights into its trehalose specificity.,Sharma D, Singh M, Kaur P, Das U Acta Crystallogr D Struct Biol. 2022 Jul 1;78(Pt 7):835-845. doi:, 10.1107/S2059798322005290. Epub 2022 Jun 7. PMID:35775983<ref>PMID:35775983</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7wcj" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Das, U]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Sharma, D]]
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[[Category: Das U]]
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[[Category: Lpqy]]
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[[Category: Sharma D]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Sugabc]]
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[[Category: Sugar binding protein]]
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Revision as of 06:27, 8 September 2022

Crystal structure LpqY from Mycobacterium tuberculosis

PDB ID 7wcj

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