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| | <StructureSection load='3u0r' size='340' side='right'caption='[[3u0r]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='3u0r' size='340' side='right'caption='[[3u0r]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3u0r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U0R FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3u0r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U0R FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">API5, MIG8 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u0r OCA], [https://pdbe.org/3u0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u0r RCSB], [https://www.ebi.ac.uk/pdbsum/3u0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u0r ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u0r OCA], [https://pdbe.org/3u0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u0r RCSB], [https://www.ebi.ac.uk/pdbsum/3u0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u0r ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/API5_HUMAN API5_HUMAN]] Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.<ref>PMID:10780674</ref> <ref>PMID:17112319</ref> <ref>PMID:19387494</ref>
| + | [https://www.uniprot.org/uniprot/API5_HUMAN API5_HUMAN] Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.<ref>PMID:10780674</ref> <ref>PMID:17112319</ref> <ref>PMID:19387494</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Apoptosis inhibitor 5 (API5) is an anti-apoptotic protein that is up-regulated in various cancer cells. Here, we present the crystal structure of human API5. API5 exhibits an elongated all alpha-helical structure. The N-terminal half of API5 is similar to the HEAT repeat and the C-terminal half is similar to the ARM (Armadillo-like) repeat. HEAT and ARM repeats have been implicated in protein-protein interactions, suggesting that the cellular roles of API5 may be to mediate protein-protein interactions. Various components of multiprotein complexes have been identified as API5-interacting protein partners, suggesting that API5 may act as a scaffold for multiprotein complexes. API5 exists as a monomer, and the functionally important heptad leucine repeat does not exhibit the predicted a dimeric leucine zipper. Additionally, Lys-251, which can be acetylated in cells, plays important roles in the inhibition of apoptosis under serum deprivation conditions. The acetylation of this lysine also affects the stability of API5 in cells.
| + | |
| - | | + | |
| - | Helical repeat structure of apoptosis inhibitor 5 reveals protein-protein interaction modules.,Han BG, Kim KH, Lee SJ, Jeong KC, Cho JW, Noh KH, Kim TW, Kim SJ, Yoon HJ, Suh SW, Lee S, Lee BI J Biol Chem. 2012 Mar 30;287(14):10727-37. doi: 10.1074/jbc.M111.317594. Epub, 2012 Feb 12. PMID:22334682<ref>PMID:22334682</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3u0r" style="background-color:#fffaf0;"></div>
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cho, J W]] | + | [[Category: Cho JW]] |
| - | [[Category: Han, B G]] | + | [[Category: Han BG]] |
| - | [[Category: Jeong, K C]] | + | [[Category: Jeong KC]] |
| - | [[Category: Kim, K H]] | + | [[Category: Kim KH]] |
| - | [[Category: Kim, T W]] | + | [[Category: Kim TW]] |
| - | [[Category: Lee, B I]] | + | [[Category: Lee BI]] |
| - | [[Category: Lee, S H]] | + | [[Category: Lee SH]] |
| - | [[Category: Noh, K H]] | + | [[Category: Noh KH]] |
| - | [[Category: Suh, S W]] | + | [[Category: Suh SW]] |
| - | [[Category: Yoon, H J]] | + | [[Category: Yoon HJ]] |
| - | [[Category: Apoptosis inhibitor]]
| + | |
| - | [[Category: Armadillo repeat]]
| + | |
| - | [[Category: Heat repeat]]
| + | |
| - | [[Category: Lysine acetylation]]
| + | |
| Structural highlights
Function
API5_HUMAN Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.[1] [2] [3]
References
- ↑ Kim JW, Cho HS, Kim JH, Hur SY, Kim TE, Lee JM, Kim IK, Namkoong SE. AAC-11 overexpression induces invasion and protects cervical cancer cells from apoptosis. Lab Invest. 2000 Apr;80(4):587-94. PMID:10780674
- ↑ Morris EJ, Michaud WA, Ji JY, Moon NS, Rocco JW, Dyson NJ. Functional identification of Api5 as a suppressor of E2F-dependent apoptosis in vivo. PLoS Genet. 2006 Nov 17;2(11):e196. PMID:17112319 doi:06-PLGE-RA-0285R2
- ↑ Rigou P, Piddubnyak V, Faye A, Rain JC, Michel L, Calvo F, Poyet JL. The antiapoptotic protein AAC-11 interacts with and regulates Acinus-mediated DNA fragmentation. EMBO J. 2009 Jun 3;28(11):1576-88. doi: 10.1038/emboj.2009.106. Epub 2009 Apr 23. PMID:19387494 doi:10.1038/emboj.2009.106
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