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3u50

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<StructureSection load='3u50' size='340' side='right'caption='[[3u50]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='3u50' size='340' side='right'caption='[[3u50]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3u50]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetth Tetth]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U50 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U50 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3u50]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U50 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U50 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3u4v|3u4v]], [[3u4z|3u4z]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TAP82 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5911 TETTH])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u50 OCA], [https://pdbe.org/3u50 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u50 RCSB], [https://www.ebi.ac.uk/pdbsum/3u50 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u50 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u50 OCA], [https://pdbe.org/3u50 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u50 RCSB], [https://www.ebi.ac.uk/pdbsum/3u50 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u50 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/TEB1_TETTS TEB1_TETTS] Single-stranded DNA (ssDNA)-binding protein that mediates the recruitment of telomerase to telomeric DNA (PubMed:19941821, PubMed:20363756, PubMed:25225329, PubMed:22143754). Telomerase is an essential ribonucleoprotein (RNP) enzyme that copies new telomeric repeats onto chromosome ends by repetitively synthesizing the short telomere-repeat sequence 5'-TTGGGG-3' using an RNA template component TER (PubMed:19941821). Acts as part of a replication protein A (RPA)-related subcomplex of the holoenzyme telomerase ribonucleoprotein complex: TEB1 specifically recognizes and binds telomeric ssDNA, thereby mediating the recruitment of the holoenzyme telomerase RNP complex to telomeres (PubMed:19941821, PubMed:25225329, PubMed:27895115). TEB1 is related to RPA1 subunit of the RPA complex but is specific to telomeric DNA, which is not the case of RPA1 (PubMed:25225329).<ref>PMID:19941821</ref> <ref>PMID:20363756</ref> <ref>PMID:22143754</ref> <ref>PMID:25225329</ref> <ref>PMID:27895115</ref>
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Telomerase copies its internal RNA template to synthesize telomeric DNA repeats. Unlike other polymerases, telomerase can retain its single-stranded product through multiple rounds of template dissociation and repositioning to accomplish repeat addition processivity (RAP). Tetrahymena telomerase holoenzyme RAP depends on a subunit, Teb1, with autonomous DNA-binding activity. Sequence homology and domain modeling suggest that Teb1 is a paralog of RPA70C, the largest subunit of the single-stranded DNA-binding factor replication protein (RPA), but unlike RPA, Teb1 binds DNA with high specificity for telomeric repeats. To understand the structural basis and significance of telomeric-repeat DNA recognition by Teb1, we solved crystal structures of three proposed Teb1 DNA-binding domains and defined amino acids of each domain that contribute to DNA interaction. Our studies indicate that two central Teb1 DNA-binding oligonucleotide/oligosaccharide-binding-fold domains, Teb1A and Teb1B, achieve high affinity and selectivity of telomeric-repeat recognition by principles similar to the telomere end-capping protein POT1 (protection of telomeres 1). An additional C-terminal Teb1 oligonucleotide/oligosaccharide-binding-fold domain, Teb1C, has features shared with the RPA70 C-terminal domain including a putative direct DNA-binding surface that is critical for high-RAP activity of reconstituted holoenzyme. The Teb1C zinc ribbon motif does not contribute to DNA binding but is nonetheless required for high-RAP activity, perhaps contributing to Teb1 physical association with the remainder of the holoenzyme. Our results suggest the biological model that high-affinity DNA binding by Teb1AB recruits holoenzyme to telomeres and subsequent Teb1C-DNA association traps product in a sliding-clamp-like manner that does not require high-affinity DNA binding for high stability of enzyme-product association.
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Structural basis for Tetrahymena telomerase processivity factor Teb1 binding to single-stranded telomeric-repeat DNA.,Zeng Z, Min B, Huang J, Hong K, Yang Y, Collins K, Lei M Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20357-61. Epub 2011 Dec 5. PMID:22143754<ref>PMID:22143754</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3u50" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Tetth]]
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[[Category: Tetrahymena thermophila]]
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[[Category: Huang, J]]
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[[Category: Huang J]]
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[[Category: Lei, M]]
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[[Category: Lei M]]
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[[Category: Yang, Y]]
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[[Category: Yang Y]]
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[[Category: Zeng, Z]]
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[[Category: Zeng Z]]
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[[Category: Dna binding protein]]
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[[Category: Processivity factor]]
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[[Category: Teb1]]
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[[Category: Telomerase]]
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[[Category: Tetrahymena]]
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Current revision

Crystal Structure of the Tetrahymena telomerase processivity factor Teb1 OB-C

PDB ID 3u50

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