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1hpu

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{{STRUCTURE_1hpu| PDB=1hpu | SCENE= }}
{{STRUCTURE_1hpu| PDB=1hpu | SCENE= }}
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'''5'-NUCLEOTIDASE (CLOSED FORM), COMPLEX WITH AMPCP'''
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===5'-NUCLEOTIDASE (CLOSED FORM), COMPLEX WITH AMPCP===
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==Overview==
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5'-Nucleotidase belongs to a large superfamily of distantly related dinuclear metallophosphatases including the Ser/Thr protein phosphatases and purple acid phosphatases. The protein undergoes a 96 degrees domain rotation between an open (inactive) and a closed (active) enzyme form. Complex structures of the closed form with the products adenosine and phosphate, and with the substrate analogue inhibitor alpha,beta-methylene ADP, have been determined at 2.1 A and 1.85 A resolution, respectively. In addition, a complex of the open form of 5'-nucleotidase with ATP was analyzed at a resolution of 1.7 A. These structures show that the adenosine group binds to a specific binding pocket of the C-terminal domain. The adenine ring is stacked between Phe429 and Phe498. The N-terminal domain provides the ligands to the dimetal cluster and the conserved His117, which together form the catalytic core structure. However, the three C-terminal arginine residues 375, 379 and 410, which are involved in substrate binding, may also play a role in transition-state stabilization. The beta-phosphate group of the inhibitor is terminally coordinated to the site 2 metal ion. The site 1 metal ion coordinates a water molecule which is in an ideal position for a nucleophilic attack on the phosphorus atom, assuming an in-line mechanism of phosphoryl transfer. Another water molecule bridges the two metal ions.
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(as it appears on PubMed at http://www.pubmed.gov), where 11491293 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11491293}}
==About this Structure==
==About this Structure==
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[[Category: Metalloenzyme]]
[[Category: Metalloenzyme]]
[[Category: Metallophosphatase]]
[[Category: Metallophosphatase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:06:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:30:48 2008''

Revision as of 05:30, 1 July 2008

Image:1hpu.png

Template:STRUCTURE 1hpu

5'-NUCLEOTIDASE (CLOSED FORM), COMPLEX WITH AMPCP

Template:ABSTRACT PUBMED 11491293

About this Structure

1HPU is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Mechanism of hydrolysis of phosphate esters by the dimetal center of 5'-nucleotidase based on crystal structures., Knofel T, Strater N, J Mol Biol. 2001 May 25;309(1):239-54. PMID:11491293

Page seeded by OCA on Tue Jul 1 08:30:48 2008

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