7xq5
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | ==== | + | ==Crystal structure of ScIno2p-ScIno4p bound promoter DNA== |
| - | <StructureSection load='7xq5' size='340' side='right'caption='[[7xq5]]' scene=''> | + | <StructureSection load='7xq5' size='340' side='right'caption='[[7xq5]], [[Resolution|resolution]] 2.25Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7xq5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XQ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XQ5 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xq5 OCA], [https://pdbe.org/7xq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xq5 RCSB], [https://www.ebi.ac.uk/pdbsum/7xq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xq5 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xq5 OCA], [https://pdbe.org/7xq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xq5 RCSB], [https://www.ebi.ac.uk/pdbsum/7xq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xq5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/INO4_YEAST INO4_YEAST]] Transcriptional activator of phospholipid synthetic genes (such as INO1, CHO1/PSS, CHO2/PEM1, OPI3/PEM2, etc.). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Gene expression is mediated by a series of regulatory proteins, i.e., transcription factors. Under different growth conditions, the transcriptional regulation of structural genes is associated with the recognition of specific regulatory elements (REs) in promoter DNA. The manner by which transcription factors recognize distinctive REs is a key question in structural biology. Previous research has demonstrated that Ino2p/Ino4p heterodimer is associated with the transcriptional regulation of phospholipid biosynthetic genes. Mechanistically, Ino2p/Ino4p could specifically recognize the inositol/choline-responsive element (ICRE), followed by the transcription activation of the phospholipid biosynthetic gene. While the promoter DNA sequence for Ino2p has already been characterized, the structural basis for the mutual interaction between Ino2p/Ino4p and their binding interface with promoter DNA remain relatively unexplored. Here, we have determined the crystalline structure of the Ino2p(DBD)/Ino4p(DBD)/DNA ternary complex, which highlights some residues (Ino2p(His12/Glu16/Arg20/Arg44) and Ino4p(His12/Glu16/Arg19/Arg20)) associated with the sequence-specific recognition of promoter DNA. Our biochemical analysis showed that mutating these residues could completely abolish protein-DNA interaction. Despite the requirement of Ino2p and Ino4p for interprotein-DNA interaction, both proteins can still interact-even in the absence of DNA. Combined with the structural analysis, our in vitro binding analysis demonstrated that residues (Arg35, Asn65, and Gln69 of Ino2p(DBD) and Leu59 of Ino4p(DBD)) are critical for interprotein interactions. Together, these results have led to the conclusion that these residues are critical to establishing interprotein-DNA and protein-DNA mutual interactions. | ||
| + | |||
| + | Structural Analysis of Ino2p/Ino4p Mutual Interactions and Their Binding Interface with Promoter DNA.,Khan MH, Xue L, Yue J, Schuller HJ, Zhu Z, Niu L Int J Mol Sci. 2022 Jul 9;23(14). pii: ijms23147600. doi: 10.3390/ijms23147600. PMID:35886947<ref>PMID:35886947</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7xq5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Saccharomyces cerevisiae]] |
| + | [[Category: Khan MH]] | ||
| + | [[Category: Lu X]] | ||
Revision as of 05:03, 8 September 2022
Crystal structure of ScIno2p-ScIno4p bound promoter DNA
| |||||||||||
