7nwr

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<StructureSection load='7nwr' size='340' side='right'caption='[[7nwr]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='7nwr' size='340' side='right'caption='[[7nwr]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7nwr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NWR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NWR FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7nwr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NWR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NWR FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nwr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nwr OCA], [https://pdbe.org/7nwr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nwr RCSB], [https://www.ebi.ac.uk/pdbsum/7nwr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nwr ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nwr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nwr OCA], [https://pdbe.org/7nwr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nwr RCSB], [https://www.ebi.ac.uk/pdbsum/7nwr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nwr ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[[https://www.uniprot.org/uniprot/A0A139K9L9_BACT4 A0A139K9L9_BACT4]]
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Inositol lipids are ubiquitous in eukaryotes and have finely tuned roles in cellular signalling and membrane homoeostasis. In Bacteria, however, inositol lipid production is relatively rare. Recently, the prominent human gut bacterium Bacteroides thetaiotaomicron (BT) was reported to produce inositol lipids and sphingolipids, but the pathways remain ambiguous and their prevalence unclear. Here, using genomic and biochemical approaches, we investigated the gene cluster for inositol lipid synthesis in BT using a previously undescribed strain with inducible control of sphingolipid synthesis. We characterized the biosynthetic pathway from myo-inositol-phosphate (MIP) synthesis to phosphoinositol dihydroceramide, determined the crystal structure of the recombinant BT MIP synthase enzyme and identified the phosphatase responsible for the conversion of bacterially-derived phosphatidylinositol phosphate (PIP-DAG) to phosphatidylinositol (PI-DAG). In vitro, loss of inositol lipid production altered BT capsule expression and antimicrobial peptide resistance. In vivo, loss of inositol lipids decreased bacterial fitness in a gnotobiotic mouse model. We identified a second putative, previously undescribed pathway for bacterial PI-DAG synthesis without a PIP-DAG intermediate, common in Prevotella. Our results indicate that inositol sphingolipid production is widespread in host-associated Bacteroidetes and has implications for symbiosis.
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Characterization of inositol lipid metabolism in gut-associated Bacteroidetes.,Heaver SL, Le HH, Tang P, Basle A, Mirretta Barone C, Vu DL, Waters JL, Marles-Wright J, Johnson EL, Campopiano DJ, Ley RE Nat Microbiol. 2022 Jul;7(7):986-1000. doi: 10.1038/s41564-022-01152-6. Epub 2022, Jun 20. PMID:35725777<ref>PMID:35725777</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7nwr" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacteroides thetaiotaomicron]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Basle, A]]
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[[Category: Basle A]]
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[[Category: Campopiano, D]]
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[[Category: Campopiano D]]
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[[Category: Marles-Wright, J]]
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[[Category: Marles-Wright J]]
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[[Category: Tang, G]]
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[[Category: Tang G]]
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[[Category: Inositol lipid]]
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[[Category: Inositol-1-phosphate]]
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[[Category: Isomerase]]
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[[Category: Nad]]
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Revision as of 05:23, 8 September 2022

Structure of BT1526, a myo-inositol-1-phosphate synthase

PDB ID 7nwr

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