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3urg

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Current revision (08:38, 20 March 2024) (edit) (undo)
 
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<StructureSection load='3urg' size='340' side='right'caption='[[3urg]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='3urg' size='340' side='right'caption='[[3urg]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3urg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Anabaena_7120 Anabaena 7120]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3URG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3URG FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3urg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3URG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3URG FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MNB:5-MERCAPTO-2-NITRO-BENZOIC+ACID'>MNB</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.003&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2k2v|2k2v]], [[2p0p|2p0p]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MNB:5-MERCAPTO-2-NITRO-BENZOIC+ACID'>MNB</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ccbP, alr1010 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=103690 Anabaena 7120])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3urg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3urg OCA], [https://pdbe.org/3urg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3urg RCSB], [https://www.ebi.ac.uk/pdbsum/3urg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3urg ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3urg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3urg OCA], [https://pdbe.org/3urg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3urg RCSB], [https://www.ebi.ac.uk/pdbsum/3urg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3urg ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8YY42_NOSS1 Q8YY42_NOSS1]
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Obtaining crystals presented a bottleneck in the structural study of Anabaena cyanobacterial Ca(2+)-binding protein (CcbP). In this report, the promoting effect of Ellman's reagent [5,5'-dithiobis(2-nitrobenzoic acid); DTNB] on the crystallization of CcbP is described. CcbP contains one free cysteine. A quick and simple oxidation reaction with DTNB blocked the free cysteine in purified CcbP and generated a homogenous monomeric protein for crystallization. The crystal structure of DTNB-modified CcbP was determined by the single-wavelength anomalous diffraction method. Structure analysis indicated that DTNB modification facilitated crystallization of CcbP by inducing polar interactions in the crystal lattice. DTNB-mediated cysteine modification was demonstrated to have little effect on the overall structure and the Ca(2+) binding of CcbP. Thus, DTNB modification may provide a simple and general approach for protein modification to improve the success of crystallization screening.
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Ellman's reagent in promoting crystallization and structure determination of Anabaena CcbP.,Fan XX, Zhou YF, Liu X, Li LF, Su XD Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Nov 1;68(Pt 11):1409-14., doi: 10.1107/S1744309112034938. Epub 2012 Oct 30. PMID:23143261<ref>PMID:23143261</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3urg" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Anabaena 7120]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Fan, X X]]
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[[Category: Nostoc sp. PCC 7120 = FACHB-418]]
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[[Category: Liu, X]]
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[[Category: Fan XX]]
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[[Category: Su, X D]]
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[[Category: Liu X]]
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[[Category: Calcium binding protein]]
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[[Category: Su XD]]
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[[Category: Metal binding protein]]
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Current revision

The crystal structure of Anabaena CcbP

PDB ID 3urg

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