3v47

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the N-terminal fragment of zebrafish TLR5 in complex with Salmonella flagellin

Structural highlights

3v47 is a 4 chain structure with sequence from Danio rerio, Eptatretus burgeri and Salmonella enterica subsp. enterica serovar Dublin. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.47Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

B3DIN1_DANRE Q4G1L2_EPTBU

Publication Abstract from PubMed

Toll-like receptor 5 (TLR5) binding to bacterial flagellin activates signaling through the transcription factor NF-kappaB and triggers an innate immune response to the invading pathogen. To elucidate the structural basis and mechanistic implications of TLR5-flagellin recognition, we determined the crystal structure of zebrafish TLR5 (as a variable lymphocyte receptor hybrid protein) in complex with the D1/D2/D3 fragment of Salmonella flagellin, FliC, at 2.47 angstrom resolution. TLR5 interacts primarily with the three helices of the FliC D1 domain using its lateral side. Two TLR5-FliC 1:1 heterodimers assemble into a 2:2 tail-to-tail signaling complex that is stabilized by quaternary contacts of the FliC D1 domain with the convex surface of the opposing TLR5. The proposed signaling mechanism is supported by structure-guided mutagenesis and deletion analyses on CBLB502, a therapeutic protein derived from FliC.

Structural basis of TLR5-flagellin recognition and signaling.,Yoon SI, Kurnasov O, Natarajan V, Hong M, Gudkov AV, Osterman AL, Wilson IA Science. 2012 Feb 17;335(6070):859-64. PMID:22344444^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yoon SI, Kurnasov O, Natarajan V, Hong M, Gudkov AV, Osterman AL, Wilson IA. Structural basis of TLR5-flagellin recognition and signaling. Science. 2012 Feb 17;335(6070):859-64. PMID:22344444 doi:10.1126/science.1215584

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3v47"

@@ Line 3: / Line 3: @@
 <StructureSection load='3v47' size='340' side='right'caption='[[3v47]], [[Resolution|resolution]] 2.47&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3v47]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bdellostoma_burgeri Bdellostoma burgeri] and [https://en.wikipedia.org/wiki/Saldu Saldu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V47 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V47 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3v47]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio], [https://en.wikipedia.org/wiki/Eptatretus_burgeri Eptatretus burgeri] and [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Dublin Salmonella enterica subsp. enterica serovar Dublin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3V47 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3V47 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.47&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3v44|3v44]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fliC, fliC1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=98360 SALDU])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3v47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3v47 OCA], [https://pdbe.org/3v47 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3v47 RCSB], [https://www.ebi.ac.uk/pdbsum/3v47 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3v47 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/FLIC_SALDU FLIC_SALDU]] Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.
+[https://www.uniprot.org/uniprot/B3DIN1_DANRE B3DIN1_DANRE] [https://www.uniprot.org/uniprot/Q4G1L2_EPTBU Q4G1L2_EPTBU]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 30: / Line 29: @@
 __TOC__
 </StructureSection>
-[[Category: Bdellostoma burgeri]]
+[[Category: Danio rerio]]
+[[Category: Eptatretus burgeri]]
 [[Category: Large Structures]]
-[[Category: Saldu]]
+[[Category: Salmonella enterica subsp. enterica serovar Dublin]]
-[[Category: Hong, H]]
+[[Category: Hong H]]
-[[Category: Wilson, I A]]
+[[Category: Wilson IA]]
-[[Category: Yoon, S I]]
+[[Category: Yoon SI]]
-[[Category: Immune system]]
-[[Category: Innate immune receptor]]
-[[Category: Innate immunity]]
-[[Category: Leucine-rich repeat]]

3v47

From Proteopedia

Current revision

Crystal structure of the N-terminal fragment of zebrafish TLR5 in complex with Salmonella flagellin

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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