7y3x
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of BTG13 mutant (H58F)== | |
| + | <StructureSection load='7y3x' size='340' side='right'caption='[[7y3x]], [[Resolution|resolution]] 2.14Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7y3x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cercospora_sojina Cercospora sojina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Y3X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Y3X FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7y3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7y3x OCA], [https://pdbe.org/7y3x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7y3x RCSB], [https://www.ebi.ac.uk/pdbsum/7y3x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7y3x ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | This study used light-mediated comparative transcriptomics to identify the biosynthetic gene cluster of beticolin 1 in Cercospora. It contains an anthraquinone moiety and an unusual halogenated xanthone moiety connected by a bicyclo[3.2.2]nonane. During elucidation of the biosynthetic pathway of beticolin 1, a novel non-heme iron oxygenase BTG13 responsible for anthraquinone ring cleavage was discovered. More importantly, the discovery of non-heme iron oxygenase BTG13 is well supported by experimental evidence: (i) crystal structure and the inductively coupled plasma mass spectrometry revealed that its reactive site is built by an atypical iron ion coordination, where the iron ion is uncommonly coordinated by four histidine residues, an unusual carboxylated-lysine (Kcx377) and water; (ii) Kcx377 is mediated by His58 and Thr299 to modulate the catalytic activity of BTG13. Therefore, we believed this study updates our knowledge of metalloenzymes. | ||
| - | + | Discovery of the Biosynthetic Pathway of Beticolin 1 Reveals a Novel Non-Heme Iron-Dependent Oxygenase for Anthraquinone Ring Cleavage.,Hou X, Xu H, Deng Z, Yan Y, Yuan Z, Liu X, Su Z, Yang S, Zhang Y, Rao Y Angew Chem Int Ed Engl. 2022 Sep 12;61(37):e202208772. doi:, 10.1002/anie.202208772. Epub 2022 Aug 4. PMID:35862137<ref>PMID:35862137</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Hou | + | <div class="pdbe-citations 7y3x" style="background-color:#fffaf0;"></div> |
| - | [[Category: Rao | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Cercospora sojina]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Hou XD]] | ||
| + | [[Category: Rao YJ]] | ||
Revision as of 07:42, 21 September 2022
Crystal structure of BTG13 mutant (H58F)
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