3vco

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Current revision (14:24, 14 March 2024) (edit) (undo)
 
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<StructureSection load='3vco' size='340' side='right'caption='[[3vco]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
<StructureSection load='3vco' size='340' side='right'caption='[[3vco]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3vco]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VCO FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3vco]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schistosoma_mansoni Schistosoma mansoni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VCO FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.946&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DHFR, Smp_175230 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6183 Blood fluke])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vco OCA], [https://pdbe.org/3vco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vco RCSB], [https://www.ebi.ac.uk/pdbsum/3vco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vco ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vco OCA], [https://pdbe.org/3vco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vco RCSB], [https://www.ebi.ac.uk/pdbsum/3vco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vco ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/DYR_SCHMA DYR_SCHMA] Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.<ref>PMID:28288799</ref>
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The parasite Schistosoma mansoni possesses all pathways for pyrimidine biosynthesis, in which dihydrofolate reductase (DHFR), thymidylate cycle participants, is essential for nucleotide metabolism to obtain energy and structural nucleic acids. Thus, DHFRs have been widely suggested as therapeutic targets for the treatment of infectious diseases. In this study, we expressed recombinant SmDHFR in a heterologous manner to obtain structural, biochemical and kinetic information. X-ray diffraction of recombinant SmDHFR at 1.95A resolution showed that the structure exhibited the canonical DHFR fold. Isothermal titration calorimetry was used to determine the kinetic constants for NADP+ and dihydrofolate. Moreover, inhibition assays were performed using the commercial folate analogs methotrexate and aminopterin; these analogs are recognized as folate competitors and are used as chemotherapeutic agents in cancer and autoimmune diseases. This study provides information that may prove useful for the future discovery of novel drugs and for understanding these metabolic steps from this pathway of S. mansoni, thus aiding in our understanding of the function of these essential pathways for parasite metabolism.
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Structure and kinetics assays of recombinant Schistosoma mansoni dihydrofolate reductase.,Serrao VHB, Romanello L, Cassago A, de Souza JRT, Cheleski J, DeMarco R, Brandao-Neto J, Pereira HD Acta Trop. 2017 Jun;170:190-196. doi: 10.1016/j.actatropica.2017.03.007. Epub, 2017 Mar 11. PMID:28288799<ref>PMID:28288799</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3vco" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Blood fluke]]
 
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[[Category: Dihydrofolate reductase]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Cassago, A]]
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[[Category: Schistosoma mansoni]]
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[[Category: DeMarco, R]]
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[[Category: Cassago A]]
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[[Category: Pereira, H M]]
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[[Category: DeMarco R]]
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[[Category: Romanello, L]]
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[[Category: Pereira HM]]
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[[Category: Serrao, V H.B]]
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[[Category: Romanello L]]
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[[Category: Oxidoreductase]]
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[[Category: Serrao VHB]]
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[[Category: Reductase]]
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Current revision

Schistosoma mansoni Dihydrofolate reductase

PDB ID 3vco

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