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1ht4

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[[Image:1ht4.gif|left|200px]]
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{{Seed}}
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[[Image:1ht4.png|left|200px]]
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{{STRUCTURE_1ht4| PDB=1ht4 | SCENE= }}
{{STRUCTURE_1ht4| PDB=1ht4 | SCENE= }}
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'''SOLUTION STRUCTURE OF A BISTRAND ABASIC SITE LESION STAGGERED IN A 3'-ORIENTATION.'''
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===SOLUTION STRUCTURE OF A BISTRAND ABASIC SITE LESION STAGGERED IN A 3'-ORIENTATION.===
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==Overview==
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A unique characteristic of ionizing radiation and radiomimetic anticancer drugs is the induction of clustered damage: two or more DNA lesions (oxidized bases, abasic sites, or strand breaks) occurring in the same or different strands of the DNA molecule within a single turn of the helix. In spite of arising at a lower frequency than single lesions, clustered DNA damage represents an exotic challenge to the repair systems present in the cells and, in some cases, these lesions may escape detection and/or processing. To understand the structural properties of clustered DNA lesions we have prepared two oligodeoxynucleotide duplexes containing adjacent tetrahydrofuran residues (abasic site analogues), positioned one in each strand of the duplex in a 5' or 3' orientation, and determined their solution structure by NMR spectroscopy and molecular dynamics simulations. The NMR data indicate that both duplex structures are right-handed helices of high similarity outside the clustered damage site. The thermal stability of the duplexes is severely reduced by the presence of the abasic residues, especially in a 5' orientation where the melting temperature is 5 degrees C lower. The structures show remarkable differences at the lesion site where the extrahelical location of the tetrahydrofuran residues in the (AP)(2)-5'-staggered duplex contrasts with their smooth alignment along the sugar-phosphate backbone in the (AP)(2)-3'-staggered duplex.
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The line below this paragraph, {{ABSTRACT_PUBMED_11327771}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11327771 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11327771}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HT4 OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HT4 OCA].
==Reference==
==Reference==
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[[Category: Clustered lesion]]
[[Category: Clustered lesion]]
[[Category: Double helix]]
[[Category: Double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:12:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:38:33 2008''

Revision as of 05:38, 1 July 2008

Image:1ht4.png

Template:STRUCTURE 1ht4

SOLUTION STRUCTURE OF A BISTRAND ABASIC SITE LESION STAGGERED IN A 3'-ORIENTATION.

Template:ABSTRACT PUBMED 11327771

About this Structure

Full experimental information is available from OCA.

Reference

NMR characterization of clustered bistrand abasic site lesions: effect of orientation on their solution structure., Lin Z, de los Santos C, J Mol Biol. 2001 Apr 27;308(2):341-52. PMID:11327771

Page seeded by OCA on Tue Jul 1 08:38:33 2008

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