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| - | [[Image:1ht4.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1ht4| PDB=1ht4 | SCENE= }} | | {{STRUCTURE_1ht4| PDB=1ht4 | SCENE= }} |
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| - | '''SOLUTION STRUCTURE OF A BISTRAND ABASIC SITE LESION STAGGERED IN A 3'-ORIENTATION.'''
| + | ===SOLUTION STRUCTURE OF A BISTRAND ABASIC SITE LESION STAGGERED IN A 3'-ORIENTATION.=== |
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| - | ==Overview==
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| - | A unique characteristic of ionizing radiation and radiomimetic anticancer drugs is the induction of clustered damage: two or more DNA lesions (oxidized bases, abasic sites, or strand breaks) occurring in the same or different strands of the DNA molecule within a single turn of the helix. In spite of arising at a lower frequency than single lesions, clustered DNA damage represents an exotic challenge to the repair systems present in the cells and, in some cases, these lesions may escape detection and/or processing. To understand the structural properties of clustered DNA lesions we have prepared two oligodeoxynucleotide duplexes containing adjacent tetrahydrofuran residues (abasic site analogues), positioned one in each strand of the duplex in a 5' or 3' orientation, and determined their solution structure by NMR spectroscopy and molecular dynamics simulations. The NMR data indicate that both duplex structures are right-handed helices of high similarity outside the clustered damage site. The thermal stability of the duplexes is severely reduced by the presence of the abasic residues, especially in a 5' orientation where the melting temperature is 5 degrees C lower. The structures show remarkable differences at the lesion site where the extrahelical location of the tetrahydrofuran residues in the (AP)(2)-5'-staggered duplex contrasts with their smooth alignment along the sugar-phosphate backbone in the (AP)(2)-3'-staggered duplex.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11327771}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11327771 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11327771}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HT4 OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HT4 OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Clustered lesion]] | | [[Category: Clustered lesion]] |
| | [[Category: Double helix]] | | [[Category: Double helix]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:12:15 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:38:33 2008'' |
Revision as of 05:38, 1 July 2008
Template:STRUCTURE 1ht4
SOLUTION STRUCTURE OF A BISTRAND ABASIC SITE LESION STAGGERED IN A 3'-ORIENTATION.
Template:ABSTRACT PUBMED 11327771
About this Structure
Full experimental information is available from OCA.
Reference
NMR characterization of clustered bistrand abasic site lesions: effect of orientation on their solution structure., Lin Z, de los Santos C, J Mol Biol. 2001 Apr 27;308(2):341-52. PMID:11327771
Page seeded by OCA on Tue Jul 1 08:38:33 2008
