7y3a
From Proteopedia
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==Crystal structure of TRIM7 bound to 2C== | ==Crystal structure of TRIM7 bound to 2C== | ||
| - | <StructureSection load='7y3a' size='340' side='right'caption='[[7y3a]]' scene=''> | + | <StructureSection load='7y3a' size='340' side='right'caption='[[7y3a]], [[Resolution|resolution]] 1.70Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Y3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Y3A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7y3a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Y3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Y3A FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7y3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7y3a OCA], [https://pdbe.org/7y3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7y3a RCSB], [https://www.ebi.ac.uk/pdbsum/7y3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7y3a ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7y3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7y3a OCA], [https://pdbe.org/7y3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7y3a RCSB], [https://www.ebi.ac.uk/pdbsum/7y3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7y3a ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/TRIM7_HUMAN TRIM7_HUMAN]] E3 ubiquitin-protein ligase. Mediates 'Lys-63'-linked polyubiquitination and stabilization of the JUN coactivator RNF187 in response to growth factor signaling via the MEK/ERK pathway, thereby regulating JUN transactivation and cellular proliferation (PubMed:25851810). Promotes the TLR4-mediated signaling activation through its E3 ligase domain leading to production of proinflammatory cytokines and type I interferon (By similarity). Plays also a negative role in the regulation of exogenous cytosolic DNA virus-triggered immune response. Mechanistically, enhances the 'Lys-48'-linked ubiquitination of STING1 leading to its proteasome-dependent degradation (PubMed:32126128).[UniProtKB:Q923T7]<ref>PMID:25851810</ref> <ref>PMID:32126128</ref> (Microbial infection) Promotes Zika virus replication by mediating envelope protein E ubiquitination.<ref>PMID:32641828</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The exposed N-terminal or C-terminal residues of proteins can act, in cognate sequence contexts, as degradation signals (degrons) that are targeted by specific E3 ubiquitin ligases for proteasome-dependent degradation by N-degron or C-degron pathways. Here, we discovered a distinct C-degron pathway, termed the Gln/C-degron pathway, in which the B30.2 domain of E3 ubiquitin ligase TRIM7 (TRIM7(B30.2)) mediates the recognition of proteins bearing a C-terminal glutamine. By determining crystal structures of TRIM7(B30.2) in complexes with various peptides, we show that TRIM7(B30.2) forms a positively charged binding pocket to engage the "U"-shaped Gln/C-degron. The four C-terminal residues of a substrate play an important role in C-degron recognition, with C-terminal glutamine as the principal determinant. In vitro biochemical and cellular experiments were used to further analyze the substrate specificity and selective degradation of the Gln/C-degron by TRIM7. | ||
| + | |||
| + | C-terminal glutamine acts as a C-degron targeted by E3 ubiquitin ligase TRIM7.,Ru Y, Yan X, Zhang B, Song L, Feng Q, Ye C, Zhou Z, Yang Z, Li Y, Zhang Z, Li Q, Mi W, Dong C Proc Natl Acad Sci U S A. 2022 Jul 26;119(30):e2203218119. doi:, 10.1073/pnas.2203218119. Epub 2022 Jul 22. PMID:35867826<ref>PMID:35867826</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7y3a" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Dong C]] | [[Category: Dong C]] | ||
[[Category: Yan X]] | [[Category: Yan X]] | ||
Revision as of 03:24, 8 September 2022
Crystal structure of TRIM7 bound to 2C
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