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7u37

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==Solution NMR structure of Vibrio cholerae ferrous iron transport protein C (FeoC)==
==Solution NMR structure of Vibrio cholerae ferrous iron transport protein C (FeoC)==
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<StructureSection load='7u37' size='340' side='right'caption='[[7u37]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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<StructureSection load='7u37' size='340' side='right'caption='[[7u37]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7u37]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U37 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U37 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7u37]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1 Vibrio cholerae O1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U37 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U37 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u37 OCA], [https://pdbe.org/7u37 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u37 RCSB], [https://www.ebi.ac.uk/pdbsum/7u37 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u37 ProSAT]</span></td></tr>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u37 OCA], [https://pdbe.org/7u37 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u37 RCSB], [https://www.ebi.ac.uk/pdbsum/7u37 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u37 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[[https://www.uniprot.org/uniprot/A0A0F0B4M5_VIBCL A0A0F0B4M5_VIBCL]]
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Most pathogenic bacteria require ferrous iron (Fe(2+)) in order to sustain infection within hosts. The ferrous iron transport (Feo) system is the most highly conserved prokaryotic transporter of Fe(2+), but its mechanism remains to be fully characterized. Most Feo systems are composed of two proteins: FeoA, a soluble SH3-like accessory protein, and FeoB, a membrane protein that translocates Fe(2+) across a lipid bilayer. Some bacterial feo operons encode FeoC, a third soluble, winged-helix protein that remains enigmatic in function. We previously demonstrated that selected FeoC proteins bind O2-sensitive [4Fe-4S] clusters via Cys residues, leading to the proposal that some FeoCs could sense O2 to regulate Fe(2+) transport. However, not all FeoCs conserve these Cys residues, and FeoC from the causative agent of cholera (Vibrio cholerae) notably lacks any Cys residues, precluding cluster binding. In this work, we determined the NMR structure of VcFeoC, which is monomeric and conserves the helix-turn-helix domain seen in other FeoCs. In contrast, however, the structure of VcFeoC reveals a truncated winged beta-sheet in which the cluster-binding domain is notably absent. Using homology modeling, we predicted the structure of VcNFeoB and used docking to identify an interaction site with VcFeoC, which is confirmed by NMR spectroscopy. These findings provide the first atomic-level structure of VcFeoC and contribute to a better understanding of its role vis-a-vis FeoB.
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The structure of Vibrio cholerae FeoC reveals conservation of the helix-turn-helix motif but not the cluster-binding domain.,Brown JB, Lee MA, Smith AT J Biol Inorg Chem. 2022 Jul 7. pii: 10.1007/s00775-022-01945-4. doi:, 10.1007/s00775-022-01945-4. PMID:35796835<ref>PMID:35796835</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7u37" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Brown, J B]]
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[[Category: Vibrio cholerae O1]]
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[[Category: Lee, M A]]
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[[Category: Brown JB]]
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[[Category: Smith, A T]]
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[[Category: Lee MA]]
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[[Category: Cytosolic protein]]
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[[Category: Smith AT]]
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[[Category: Feo]]
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[[Category: Feoc]]
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[[Category: Ferrous iron transport]]
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Revision as of 16:49, 7 September 2022

Solution NMR structure of Vibrio cholerae ferrous iron transport protein C (FeoC)

PDB ID 7u37

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