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1hv6

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{{STRUCTURE_1hv6| PDB=1hv6 | SCENE= }}
{{STRUCTURE_1hv6| PDB=1hv6 | SCENE= }}
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'''CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III COMPLEXED WITH TRISACCHARIDE PRODUCT.'''
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===CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III COMPLEXED WITH TRISACCHARIDE PRODUCT.===
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==Overview==
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The structure of A1-III from a Sphingomonas species A1 complexed with a trisaccharide product (4-deoxy-l-erythro-hex-4-enepyranosyluronate-mannuronate-mannuronic acid) was determined by X-ray crystallography at 2.0 A with an R-factor of 0.16. The final model of the complex form comprising 351 amino acid residues, 245 water molecules, one sulfate ion and one trisaccharide product exhibited a C(alpha) r.m.s.d. value of 0.154 A with the reported apo form of the enzyme. The trisaccharide was bound in the active cleft at subsites -3 approximately -1 from the non-reducing end by forming several hydrogen bonds and van der Waals interactions with protein atoms. The catalytic residue was estimated to be Tyr246, which existed between subsites -1 and +1 based on a mannuronic acid model oriented at subsite +1.
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(as it appears on PubMed at http://www.pubmed.gov), where 11243798 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11243798}}
==About this Structure==
==About this Structure==
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[[Category: Alginate lyase]]
[[Category: Alginate lyase]]
[[Category: Trisaccharide complex,alpha barrel]]
[[Category: Trisaccharide complex,alpha barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:15:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:49:25 2008''

Revision as of 05:49, 1 July 2008

Image:1hv6.png

Template:STRUCTURE 1hv6

CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III COMPLEXED WITH TRISACCHARIDE PRODUCT.

Template:ABSTRACT PUBMED 11243798

About this Structure

1HV6 is a Single protein structure of sequence from Sphingomonas species. Full crystallographic information is available from OCA.

Reference

Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution., Yoon HJ, Hashimoto W, Miyake O, Murata K, Mikami B, J Mol Biol. 2001 Mar 16;307(1):9-16. PMID:11243798

Page seeded by OCA on Tue Jul 1 08:49:25 2008

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