7z6m
From Proteopedia
(Difference between revisions)
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==Crystal structure of Zn2+-transporter BbZIP in a cadmium bound state== | ==Crystal structure of Zn2+-transporter BbZIP in a cadmium bound state== | ||
| - | <StructureSection load='7z6m' size='340' side='right'caption='[[7z6m]]' scene=''> | + | <StructureSection load='7z6m' size='340' side='right'caption='[[7z6m]], [[Resolution|resolution]] 2.51Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z6M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7z6m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_bronchiseptica Bordetella bronchiseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z6M FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z6m OCA], [https://pdbe.org/7z6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z6m RCSB], [https://www.ebi.ac.uk/pdbsum/7z6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z6m ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.51Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z6m OCA], [https://pdbe.org/7z6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z6m RCSB], [https://www.ebi.ac.uk/pdbsum/7z6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z6m ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0H3LM39_BORBR A0A0H3LM39_BORBR] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Zinc is essential for all organisms and yet detrimental at elevated levels. Hence, homeostasis of this metal is tightly regulated. The Zrt/Irt-like proteins (ZIPs) represent the only zinc importers in metazoans. Mutations in human ZIPs cause serious disorders, but the mechanism by which ZIPs transfer zinc remains elusive. Hitherto, structural information is only available for a model member, BbZIP, and as a single, ion-bound conformation, precluding mechanistic insights. Here, we elucidate an inward-open metal-free BbZIP structure, differing substantially in the relative positions of the two separate domains of ZIPs. With accompanying coevolutional analyses, mutagenesis, and uptake assays, the data point to an elevator-type transport mechanism, likely shared within the ZIP family, unifying earlier functional data. Moreover, the structure reveals a previously unknown ninth transmembrane segment that is important for activity in vivo. Our findings outline the mechanistic principles governing ZIP-protein transport and enhance the molecular understanding of ZIP-related disorders. | ||
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| + | The two-domain elevator-type mechanism of zinc-transporting ZIP proteins.,Wiuf A, Steffen JH, Becares ER, Gronberg C, Mahato DR, Rasmussen SGF, Andersson M, Croll T, Gotfryd K, Gourdon P Sci Adv. 2022 Jul 15;8(28):eabn4331. doi: 10.1126/sciadv.abn4331. Epub 2022 Jul, 13. PMID:35857505<ref>PMID:35857505</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7z6m" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bordetella bronchiseptica]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Andersson M]] | [[Category: Andersson M]] | ||
Current revision
Crystal structure of Zn2+-transporter BbZIP in a cadmium bound state
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