1hwp

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{{STRUCTURE_1hwp| PDB=1hwp | SCENE= }}
{{STRUCTURE_1hwp| PDB=1hwp | SCENE= }}
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'''EBULIN COMPLEXED WITH PTEROIC ACID, TRIGONAL CRYSTAL FORM'''
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===EBULIN COMPLEXED WITH PTEROIC ACID, TRIGONAL CRYSTAL FORM===
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==Overview==
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Ebulin l is a type-II ribosome-inactivating protein (RIP) isolated from the leaves of Sambucus ebulus L. As with other type-II RIP, ebulin is a disulfide-linked heterodimer composed of a toxic A chain and a galactoside-specific lectin B chain. A normal level of ribosome-inactivating N-glycosidase activity, characteristic of the A chain of type-II RIP, has been demonstrated for ebulin l. However, ebulin is considered a nontoxic type-II RIP due to a reduced cytotoxicity on whole cells and animals as compared with other toxic type-II RIP like ricin. The molecular cloning, amino acid sequence, and the crystal structure of ebulin l are presented and compared with ricin. Ebulin l is shown to bind an A-chain substrate analogue, pteroic acid, in the same manner as ricin. The galactoside-binding ability of ebulin l is demonstrated crystallographically with a complex of the B chain with galactose and with lactose. The negligible cytotoxicity of ebulin l is apparently due to a reduced affinity for galactosides. An altered mode of galactoside binding in the 2gamma subdomain of the lectin B chain primarily causes the reduced affinity.
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The line below this paragraph, {{ABSTRACT_PUBMED_11288182}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11288182 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11288182}}
==About this Structure==
==About this Structure==
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[[Category: Ribosome-inactivating protein]]
[[Category: Ribosome-inactivating protein]]
[[Category: Ricin-like]]
[[Category: Ricin-like]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:18:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:01:39 2008''

Revision as of 07:01, 1 July 2008

Image:1hwp.png

Template:STRUCTURE 1hwp

EBULIN COMPLEXED WITH PTEROIC ACID, TRIGONAL CRYSTAL FORM

Template:ABSTRACT PUBMED 11288182

About this Structure

Full crystallographic information is available from OCA.

Reference

2.8-A crystal structure of a nontoxic type-II ribosome-inactivating protein, ebulin l., Pascal JM, Day PJ, Monzingo AF, Ernst SR, Robertus JD, Iglesias R, Perez Y, Ferreras JM, Citores L, Girbes T, Proteins. 2001 May 15;43(3):319-26. PMID:11288182

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