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3wx4

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Current revision (08:47, 20 March 2024) (edit) (undo)
 
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<StructureSection load='3wx4' size='340' side='right'caption='[[3wx4]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
<StructureSection load='3wx4' size='340' side='right'caption='[[3wx4]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3wx4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WX4 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3wx4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WX4 FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">arn, asiA.1, motA.-6 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wx4 OCA], [https://pdbe.org/3wx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wx4 RCSB], [https://www.ebi.ac.uk/pdbsum/3wx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wx4 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wx4 OCA], [https://pdbe.org/3wx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wx4 RCSB], [https://www.ebi.ac.uk/pdbsum/3wx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wx4 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/ARN_BPT4 ARN_BPT4]] Strongly inhibits the RgIB endonuclease.
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[https://www.uniprot.org/uniprot/ARN_BPT4 ARN_BPT4] Strongly inhibits the RgIB endonuclease.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The T4 phage protein Arn (Anti restriction nuclease) was identified as an inhibitor of the restriction enzyme McrBC. However, until now its molecular mechanism remained unclear. In the present study we used structural approaches to investigate biological properties of Arn. A structural analysis of Arn revealed that its shape and negative charge distribution are similar to dsDNA, suggesting that this protein could act as a DNA mimic. In a subsequent proteomic analysis, we found that the bacterial histone-like protein H-NS interacts with Arn, implying a new function. An electrophoretic mobility shift assay showed that Arn prevents H-NS from binding to the Escherichia coli hns and T4 p8.1 promoters. In vitro gene expression and electron microscopy analyses also indicated that Arn counteracts the gene-silencing effect of H-NS on a reporter gene. Because McrBC and H-NS both participate in the host defense system, our findings suggest that T4 Arn might knock down these mechanisms using its DNA mimicking properties.
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The T4 phage DNA mimic protein Arn inhibits the DNA binding activity of the bacterial histone-like protein H-NS.,Ho CH, Wang HC, Ko TP, Chang YC, Wang AH J Biol Chem. 2014 Sep 26;289(39):27046-54. doi: 10.1074/jbc.M114.590851. Epub, 2014 Aug 12. PMID:25118281<ref>PMID:25118281</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3wx4" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bpt4]]
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[[Category: Escherichia virus T4]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Ho, C H]]
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[[Category: Ho CH]]
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[[Category: Ko, T P]]
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[[Category: Ko TP]]
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[[Category: Wang, A H.J]]
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[[Category: Wang AHJ]]
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[[Category: Wang, H C]]
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[[Category: Wang HC]]
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[[Category: Dna mimic]]
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[[Category: Gene regulation]]
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[[Category: Viral protein]]
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Current revision

CRYSTAL STRUCTURE of T4 PHAGE ARN PROTEIN

PDB ID 3wx4

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