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3wyr

From Proteopedia

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Crystal structure of Killer cell immunoglobulin-like receptor 2DL4

Structural highlights

3wyr is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.8Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KI2L4_HUMAN Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis.

Publication Abstract from PubMed

The engagement of natural killer cell immunoglobulin-like receptors (KIRs) with their target ligands, human leukocyte antigen (HLA) molecules, is a critical component of innate immunity. Structurally, KIRs typically have either two (D1-D2) or three (D0-D1-D2) extracellular immunoglobulin domains, with the D1 and D2 domain recognizing the alpha1 and alpha2 helices of HLA, respectively, whereas the D0 domain of the KIR3DLs binds a loop region flanking the alpha1 helix of the HLA molecule. KIR2DL4 is distinct from other KIRs (except KIR2DL5) in that it does not contain a D1 domain and instead has a D0-D2 arrangement. Functionally, KIR2DL4 is also atypical in that, unlike all other KIRs, KIR2DL4 has both activating and inhibitory signaling domains. Here, we determined the 2.8 A crystal structure of the extracellular domains of KIR2DL4. Structurally, KIR2DL4 is reminiscent of other KIR2DL receptors, with the D0 and D2 adopting the C2-type immunoglobulin fold arranged with an acute elbow angle. However, KIR2DL4 self-associated via the D0 domain in a concentration-dependent manner and was observed as a tetramer in the crystal lattice by size exclusion chromatography, dynamic light scattering, analytical ultracentrifugation, and small angle x-ray scattering experiments. The assignment of residues in the D0 domain to forming the KIR2DL4 tetramer precludes an interaction with HLA akin to that observed for KIR3DL1. Accordingly, no interaction was observed to HLA by direct binding studies. Our data suggest that the unique functional properties of KIR2DL4 may be mediated by self-association of the receptor.

The structure of the atypical killer cell immunoglobulin-like receptor, KIR2DL4.,Moradi S, Berry R, Pymm P, Hitchen C, Beckham SA, Wilce MC, Walpole NG, Clements CS, Reid HH, Perugini MA, Brooks AG, Rossjohn J, Vivian JP J Biol Chem. 2015 Apr 17;290(16):10460-71. doi: 10.1074/jbc.M114.612291. Epub, 2015 Mar 10. PMID:25759384^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Moradi S, Berry R, Pymm P, Hitchen C, Beckham SA, Wilce MC, Walpole NG, Clements CS, Reid HH, Perugini MA, Brooks AG, Rossjohn J, Vivian JP. The structure of the atypical killer cell immunoglobulin-like receptor, KIR2DL4. J Biol Chem. 2015 Apr 17;290(16):10460-71. doi: 10.1074/jbc.M114.612291. Epub, 2015 Mar 10. PMID:25759384 doi:http://dx.doi.org/10.1074/jbc.M114.612291

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3wyr"

Categories: Homo sapiens | Large Structures | Moradi S | Rossjohn J | Vivian JP

@@ Line 3: / Line 3: @@
 <StructureSection load='3wyr' size='340' side='right'caption='[[3wyr]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3wyr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WYR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3wyr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WYR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WYR FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KIR2DL4, CD158D, KIR103AS ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wyr OCA], [https://pdbe.org/3wyr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wyr RCSB], [https://www.ebi.ac.uk/pdbsum/3wyr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wyr ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/KI2L4_HUMAN KI2L4_HUMAN]] Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis.
+[https://www.uniprot.org/uniprot/KI2L4_HUMAN KI2L4_HUMAN] Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 26: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Moradi, S]]
+[[Category: Moradi S]]
-[[Category: Rossjohn, J]]
+[[Category: Rossjohn J]]
-[[Category: Vivian, J P]]
+[[Category: Vivian JP]]
-[[Category: Immune receptor]]
-[[Category: Immune system]]
-[[Category: Immunoglobulin domain]]

3wyr

From Proteopedia

Current revision

Crystal structure of Killer cell immunoglobulin-like receptor 2DL4

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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