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| | <StructureSection load='3wz0' size='340' side='right'caption='[[3wz0]], [[Resolution|resolution]] 2.79Å' scene=''> | | <StructureSection load='3wz0' size='340' side='right'caption='[[3wz0]], [[Resolution|resolution]] 2.79Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3wz0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_kodakaraensis_(strain_kod1) Pyrococcus kodakaraensis (strain kod1)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WZ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WZ0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3wz0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_kodakarensis_KOD1 Thermococcus kodakarensis KOD1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WZ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WZ0 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3wyz|3wyz]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.79Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rnp3, TK1450 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69014 Pyrococcus kodakaraensis (strain KOD1)]), rnp2, TK1767 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=69014 Pyrococcus kodakaraensis (strain KOD1)])</td></tr>
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| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ribonuclease_P Ribonuclease P], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.5 3.1.26.5] </span></td></tr>
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wz0 OCA], [https://pdbe.org/3wz0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wz0 RCSB], [https://www.ebi.ac.uk/pdbsum/3wz0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wz0 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wz0 OCA], [https://pdbe.org/3wz0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wz0 RCSB], [https://www.ebi.ac.uk/pdbsum/3wz0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wz0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/RNP3_THEKO RNP3_THEKO]] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00756] [[https://www.uniprot.org/uniprot/RNP2_THEKO RNP2_THEKO]] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00755]
| + | [https://www.uniprot.org/uniprot/RNP3_THEKO RNP3_THEKO] Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.[HAMAP-Rule:MF_00756] |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The ribonuclease P (RNase P) proteins TkoPop5 and TkoRpp30, homologs of human Pop5 and Rpp30, respectively, in the hyperthermophilic archaeon Thermococcus kodakarensis were prepared and characterized with respect to pre-tRNA cleavage activity using the reconstitution system of the well-studied Pyrococcus horikoshii RNase P. The reconstituted particle containing TkoPop5 in place of the P. horikoshii counterpart PhoPop5 retained pre-tRNA cleavage activity comparable to that of the reconstituted P. horikoshii RNase P, while that containing TkoRpp30 instead of its corresponding protein PhoRpp30 had slightly lower activity than the P. horikoshii RNase P. Moreover, we determined crystal structures of TkoRpp30 alone and in complex with TkoPop5. Like their P. horikoshii counterparts, whose structures were solved previously, TkoRpp30 and TkoPop5 fold into TIM barrel and RRM-like fold, respectively. This finding demonstrates that RNase P proteins in T. kodakarensis and P. horikoshii are interchangeable and that their three-dimensional structures are highly conserved.
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| - | | + | |
| - | On archaeal homologs of the human RNase P proteins Pop5 and Rpp30 in the hyperthermophilic archaeon Thermococcus kodakarensis.,Suematsu K, Ueda T, Nakashima T, Kakuta Y, Kimura M Biosci Biotechnol Biochem. 2015;79(6):952-9. doi: 10.1080/09168451.2014.1003130. , Epub 2015 Feb 23. PMID:25704799<ref>PMID:25704799</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3wz0" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ribonuclease P]] | + | [[Category: Thermococcus kodakarensis KOD1]] |
| - | [[Category: Kakuta, Y]] | + | [[Category: Kakuta Y]] |
| - | [[Category: Kimura, M]] | + | [[Category: Kimura M]] |
| - | [[Category: Nakashima, T]] | + | [[Category: Nakashima T]] |
| - | [[Category: Suematsu, K]] | + | [[Category: Suematsu K]] |
| - | [[Category: Ueda, T]] | + | [[Category: Ueda T]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Pre-trna cleavage]]
| + | |
| - | [[Category: Rrm-like structure]]
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| - | [[Category: Tim barrel-like structure]]
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