7n6o
From Proteopedia
(Difference between revisions)
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==The crystal structure of the GH30 subfamily 10 enzyme, AcXbh30A from Acetivibrio clariflavus in complex with xylobiose== | ==The crystal structure of the GH30 subfamily 10 enzyme, AcXbh30A from Acetivibrio clariflavus in complex with xylobiose== | ||
| - | <StructureSection load='7n6o' size='340' side='right'caption='[[7n6o]]' scene=''> | + | <StructureSection load='7n6o' size='340' side='right'caption='[[7n6o]], [[Resolution|resolution]] 1.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N6O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N6O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7n6o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_clariflavus_DSM_19732 Acetivibrio clariflavus DSM 19732]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7N6O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7N6O FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n6o OCA], [https://pdbe.org/7n6o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n6o RCSB], [https://www.ebi.ac.uk/pdbsum/7n6o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n6o ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PRD_900116:4beta-beta-xylobiose'>PRD_900116</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7n6o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7n6o OCA], [https://pdbe.org/7n6o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7n6o RCSB], [https://www.ebi.ac.uk/pdbsum/7n6o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7n6o ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/G8LU16_ACECE G8LU16_ACECE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Xylobiose is a prebiotic sugar that has applications in functional foods. This report describes the first X-ray crystallographic structure models of apo and xylobiose-bound forms of a xylobiohydrolase (XBH) from Acetivibrio clariflavus. This xylan-active enzyme, a member of the recently described glycoside hydrolase family 30 (GH30), subfamily 10, phylogenetic clade has been shown to strictly release xylobiose as its primary hydrolysis product. Inspection of the apo structure reveals a glycone region X2 -binding slot. When X2 binds, the non-reducing xylose in the -2 subsite is highly coordinated with numerous hydrogen bond contacts while contacts in the -1 subsite mostly reflect interactions typical for GH30 and enzymes in clan A of the carbohydrate-active enzymes database (CAZy). This structure provides an explanation for the high functional specificity of this new bacterial GH30 XBH subfamily. | ||
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| + | The first crystal structure of a xylobiose-bound xylobiohydrolase with high functional specificity from the bacterial glycoside hydrolase family 30, subfamily 10.,St John FJ, Crooks C, Kim Y, Tan K, Joachimiak A FEBS Lett. 2022 Jul 25. doi: 10.1002/1873-3468.14454. PMID:35876256<ref>PMID:35876256</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7n6o" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Acetivibrio clariflavus DSM 19732]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: St John JF]] | [[Category: St John JF]] | ||
[[Category: Tan K]] | [[Category: Tan K]] | ||
Current revision
The crystal structure of the GH30 subfamily 10 enzyme, AcXbh30A from Acetivibrio clariflavus in complex with xylobiose
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