7ut7
From Proteopedia
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| - | ==== | + | ==C2 symmetric cryoEM structure of Azotobacter vinelandii MoFeP under non-turnover conditions== |
| - | <StructureSection load='7ut7' size='340' side='right'caption='[[7ut7]]' scene=''> | + | <StructureSection load='7ut7' size='340' side='right'caption='[[7ut7]], [[Resolution|resolution]] 1.91Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ut7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii_DJ Azotobacter vinelandii DJ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UT7 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ut7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ut7 OCA], [https://pdbe.org/7ut7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ut7 RCSB], [https://www.ebi.ac.uk/pdbsum/7ut7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ut7 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLF:FE(8)-S(7)+CLUSTER'>CLF</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HCA:3-HYDROXY-3-CARBOXY-ADIPIC+ACID'>HCA</scene>, <scene name='pdbligand=ICS:IRON-SULFUR-MOLYBDENUM+CLUSTER+WITH+INTERSTITIAL+CARBON'>ICS</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ut7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ut7 OCA], [https://pdbe.org/7ut7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ut7 RCSB], [https://www.ebi.ac.uk/pdbsum/7ut7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ut7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/NIFD_AZOVI NIFD_AZOVI]] This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The enzyme nitrogenase couples adenosine triphosphate (ATP) hydrolysis to the multielectron reduction of atmospheric dinitrogen into ammonia. Despite extensive research, the mechanistic details of ATP-dependent energy transduction and dinitrogen reduction by nitrogenase are not well understood, requiring new strategies to monitor its structural dynamics during catalytic action. Here, we report cryo-electron microscopy structures of the nitrogenase complex prepared under enzymatic turnover conditions. We observe that asymmetry governs all aspects of the nitrogenase mechanism, including ATP hydrolysis, protein-protein interactions, and catalysis. Conformational changes near the catalytic iron-molybdenum cofactor are correlated with the nucleotide-hydrolysis state of the enzyme. | ||
| + | |||
| + | Structures of the nitrogenase complex prepared under catalytic turnover conditions.,Rutledge HL, Cook BD, Nguyen HPM, Herzik MA Jr, Tezcan FA Science. 2022 Aug 19;377(6608):865-869. doi: 10.1126/science.abq7641. Epub 2022, Jul 28. PMID:35901182<ref>PMID:35901182</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ut7" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Azotobacter vinelandii DJ]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Cook BD]] |
| + | [[Category: Herzik MA]] | ||
| + | [[Category: Rutledge HL]] | ||
| + | [[Category: Tezcan FA]] | ||
Revision as of 19:51, 7 September 2022
C2 symmetric cryoEM structure of Azotobacter vinelandii MoFeP under non-turnover conditions
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