This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4a0u

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Structure of the carboxy-terminal domain of bacteriophage T7 fibre gp17 containing residues 371-553, C2221 crystal form.

Structural highlights

4a0u is a 3 chain structure with sequence from Escherichia phage T7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FIBER_BPT7 Structural component of the short non-contractile tail. The tail comprises six fibers made of gp17 trimers, 2 nm in diameter and 32 nm in length. May attach to host lipopolysaccharides (LPS) to mediate primary attachment to the host cell.^[1]

Publication Abstract from PubMed

The six bacteriophage T7 tail fibers, homo-trimers of gene product 17, are thought to be responsible for the first specific, albeit reversible, attachment to Escherichia coli lipopolysaccharide. The protein trimer forms kinked fibers comprised of an amino-terminal tail-attachment domain, a slender shaft, and a carboxyl-terminal domain composed of several nodules. Previously, we expressed, purified, and crystallized a carboxyl-terminal fragment comprising residues 371-553. Here, we report the structure of this protein trimer, solved using anomalous diffraction and refined at 2 A resolution. Amino acids 371-447 form a tapered pyramid with a triangular cross-section composed of interlocked beta-sheets from each of the three chains. The triangular pyramid domain has three alpha-helices at its narrow end, which are connected to a carboxyl-terminal three-blade beta-propeller tip domain by flexible loops. The monomers of this tip domain each contain an eight-stranded beta-sandwich. The exact topology of the beta-sandwich fold is novel, but similar to that of knob domains of other viral fibers and the phage Sf6 needle. Several host-range change mutants have been mapped to loops located on the top of this tip domain, suggesting that this surface of the tip domain interacts with receptors on the cell surface.

Structure of the receptor-binding carboxy-terminal domain of bacteriophage T7 tail fibers.,Garcia-Doval C, van Raaij MJ Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9390-5. Epub 2012 May 29. PMID:22645347^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cuervo A, Pulido-Cid M, Chagoyen M, Arranz R, Gonzalez-Garcia VA, Garcia-Doval C, Caston JR, Valpuesta JM, van Raaij MJ, Martin-Benito J, Carrascosa JL. Structural Characterization Of The Bacteriophage T7 Tail Machinery. J Biol Chem. 2013 Jul 24. PMID:23884409 doi:10.1074/jbc.M113.491209
↑ Garcia-Doval C, van Raaij MJ. Structure of the receptor-binding carboxy-terminal domain of bacteriophage T7 tail fibers. Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9390-5. Epub 2012 May 29. PMID:22645347 doi:10.1073/pnas.1119719109

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4a0u"

Categories: Escherichia phage T7 | Large Structures | Garcia-Doval C | Van Raaij MJ

@@ Line 3: / Line 3: @@
 <StructureSection load='4a0u' size='340' side='right'caption='[[4a0u]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4a0u]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A0U FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4a0u]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A0U FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4a0t|4a0t]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a0u OCA], [https://pdbe.org/4a0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a0u RCSB], [https://www.ebi.ac.uk/pdbsum/4a0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a0u ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/FIBER_BPT7 FIBER_BPT7]] Structural component of the short non-contractile tail. The tail comprises six fibers made of gp17 trimers, 2 nm in diameter and 32 nm in length. May attach to host lipopolysaccharides (LPS) to mediate primary attachment to the host cell.<ref>PMID:23884409</ref>
+[https://www.uniprot.org/uniprot/FIBER_BPT7 FIBER_BPT7] Structural component of the short non-contractile tail. The tail comprises six fibers made of gp17 trimers, 2 nm in diameter and 32 nm in length. May attach to host lipopolysaccharides (LPS) to mediate primary attachment to the host cell.<ref>PMID:23884409</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Bpt7]]
+[[Category: Escherichia phage T7]]
 [[Category: Large Structures]]
-[[Category: Garcia-Doval, C]]
+[[Category: Garcia-Doval C]]
-[[Category: Raaij, M J.van]]
+[[Category: Van Raaij MJ]]
-[[Category: Bacteriophage fibre]]
-[[Category: Beta-helix]]
-[[Category: Beta-sandwich]]
-[[Category: Caudovirale]]
-[[Category: Podoviridae]]
-[[Category: Receptor binding]]
-[[Category: Viral protein]]

4a0u

From Proteopedia

Current revision

Structure of the carboxy-terminal domain of bacteriophage T7 fibre gp17 containing residues 371-553, C2221 crystal form.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox