7te2
From Proteopedia
(Difference between revisions)
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==Crystal Structure of AerR from Rhodobacter capsulatus at 2.25 A.== | ==Crystal Structure of AerR from Rhodobacter capsulatus at 2.25 A.== | ||
| - | <StructureSection load='7te2' size='340' side='right'caption='[[7te2]]' scene=''> | + | <StructureSection load='7te2' size='340' side='right'caption='[[7te2]], [[Resolution|resolution]] 2.25Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TE2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7te2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodobacter_capsulatus_Y262 Rhodobacter capsulatus Y262]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TE2 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7te2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7te2 OCA], [https://pdbe.org/7te2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7te2 RCSB], [https://www.ebi.ac.uk/pdbsum/7te2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7te2 ProSAT]</span></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7te2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7te2 OCA], [https://pdbe.org/7te2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7te2 RCSB], [https://www.ebi.ac.uk/pdbsum/7te2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7te2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/A0A829NYX9_RHOCA A0A829NYX9_RHOCA]] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Among purple photosynthetic bacteria, the transcription factor CrtJ is a major regulator of photosystem gene expression. Depending on growing conditions, CrtJ can function as an aerobic repressor or an anaerobic activator of photosystem genes. Recently, CrtJ's activity was shown to be modulated by two size variants of a B12 binding co-regulator called SAerR and LAerR in Rhodobacter capsulatus. The short form, SAerR, promotes CrtJ repression, while the longer variant, LAerR, converts CrtJ into an activator. In this study, we solved the crystal structure of R. capsulatus SAerR at a 2.25 A resolution. Hydroxycobalamin bound to SAerR is sandwiched between a 4-helix bundle cap, and a Rossman fold. This structure is similar to a AerR-like domain present in CarH from Thermus termophilus, which is a combined photoreceptor/transcription regulator. We also utilized AlphaFold software to predict structures for the LAerR, CrtJ, SAerR-CrtJ and LAerR-CrtJ co-complexes. These structures provide insights into the role of B12 and an LAerR N-terminal extension in regulating the activity of CrtJ. | ||
| + | |||
| + | Structural Analyses of CrtJ and Its B12-Binding Co-Regulators SAerR and LAerR from the Purple Photosynthetic Bacterium Rhodobacter capsulatus.,Dragnea V, Gonzalez-Gutierrez G, Bauer CE Microorganisms. 2022 Apr 27;10(5). pii: microorganisms10050912. doi:, 10.3390/microorganisms10050912. PMID:35630357<ref>PMID:35630357</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7te2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Rhodobacter capsulatus Y262]] | ||
[[Category: Bauer CE]] | [[Category: Bauer CE]] | ||
[[Category: Dragnea V]] | [[Category: Dragnea V]] | ||
[[Category: Gonzalez-Gutierrez G]] | [[Category: Gonzalez-Gutierrez G]] | ||
Revision as of 19:25, 7 September 2022
Crystal Structure of AerR from Rhodobacter capsulatus at 2.25 A.
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