7v9u
From Proteopedia
(Difference between revisions)
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| - | ==== | + | ==Cryo-EM structure of E.coli retron-Ec86 (RT-msDNA-RNA) at 3.2 angstrom== |
| - | <StructureSection load='7v9u' size='340' side='right'caption='[[7v9u]]' scene=''> | + | <StructureSection load='7v9u' size='340' side='right'caption='[[7v9u]], [[Resolution|resolution]] 3.12Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7v9u]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V9U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V9U FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v9u OCA], [https://pdbe.org/7v9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v9u RCSB], [https://www.ebi.ac.uk/pdbsum/7v9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v9u ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v9u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v9u OCA], [https://pdbe.org/7v9u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v9u RCSB], [https://www.ebi.ac.uk/pdbsum/7v9u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v9u ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/RT86_ECOLX RT86_ECOLX]] Reverse transcriptase (RT) component of antiviral defense system retron Ec86, composed of a non-coding RNA (ncRNA), a ribosyltransferase/DNA-binding protein and this RT. Expression of the 3-gene retron confers protection against bacteriophage T5. At multiplicity of infection (MOI) of 0.02 cultures grow normally when infected with T5 without collapsing, at MOI 2 cultures enter growth stasis (PubMed:33157039). Responsible for synthesis of msDNA (a branched molecule with RNA linked by a 2',5'-phosphodiester bond to ssDNA). The retron transcript serves as primer (from a conserved internal G residue) and template for the reaction, and codes for the RT (PubMed:2466573, PubMed:10531319). Recognizes only its cognate RNA as a primer template (PubMed:10531319). Overexpression of the ncRNA and RT (without the ribosyltransferase), which leads to increased levels of msDNA, is mutagenic in vivo (PubMed:7885227). This may be due to a mismatch in the msDNA stem which binds and sequesters MutS and/or MutL (Probable).<ref>PMID:10531319</ref> <ref>PMID:2466573</ref> <ref>PMID:33157039</ref> <ref>PMID:7885227</ref> <ref>PMID:7885227</ref> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Guan ZY]] |
| + | [[Category: Wang YJ]] | ||
| + | [[Category: Zou TT]] | ||
Revision as of 19:29, 7 September 2022
Cryo-EM structure of E.coli retron-Ec86 (RT-msDNA-RNA) at 3.2 angstrom
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