4b4j

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4b4j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B4J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B4J FirstGlance]. <br>
<table><tr><td colspan='2'>[[4b4j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B4J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B4J FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4b49|4b49]], [[4b4e|4b4e]], [[4b4i|4b4i]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b4j OCA], [https://pdbe.org/4b4j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b4j RCSB], [https://www.ebi.ac.uk/pdbsum/4b4j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b4j ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b4j OCA], [https://pdbe.org/4b4j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b4j RCSB], [https://www.ebi.ac.uk/pdbsum/4b4j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b4j ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
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[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization.
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Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol.,Stauber M, Jakoncic J, Berger J, Karp JM, Axelbaum A, Sastow D, Buldyrev SV, Hrnjez BJ, Asherie N Acta Crystallogr D Biol Crystallogr. 2015 Mar 1;71(Pt 3):427-41. doi:, 10.1107/S1399004714025061. Epub 2015 Feb 26. PMID:25760593<ref>PMID:25760593</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4b4j" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Lysozyme]]
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[[Category: Asherie N]]
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[[Category: Asherie, N]]
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[[Category: Axelbaum A]]
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[[Category: Axelbaum, A]]
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[[Category: Berger J]]
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[[Category: Berger, J]]
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[[Category: Jakoncic J]]
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[[Category: Jakoncic, J]]
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[[Category: Stauber M]]
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[[Category: Stauber, M]]
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[[Category: Chirality]]
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[[Category: Hydrolase]]
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Revision as of 07:17, 1 May 2024

1.25 A Structure of Lysozyme Crystallized with (RS)-2-methyl-2,4- pentanediol

PDB ID 4b4j

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