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1i4u

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[[Image:1i4u.gif|left|200px]]
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{{STRUCTURE_1i4u| PDB=1i4u | SCENE= }}
{{STRUCTURE_1i4u| PDB=1i4u | SCENE= }}
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'''THE C1 SUBUNIT OF ALPHA-CRUSTACYANIN'''
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===THE C1 SUBUNIT OF ALPHA-CRUSTACYANIN===
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==Overview==
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The previously unknown crystal structure of the C(1) subunit of the carotenoid-binding protein alpha-crustacyanin has been determined using the anomalous scattering available at 1.77 A wavelength to determine the partial structure of the S atoms intrinsic to the native protein. The resulting 'heavy-atom' phases, in conjunction with near-atomic resolution (d(min) = 1.15 A) data, were then used to initiate successful structure determination using a direct-methods approach. This is, to the authors' knowledge, the first time such a small anomalous signal ( approximately 1%) has been used to aid the determination of a macromolecular structure. As well as the structure itself, the methods used during data collection and those used in the elucidation of the sulfur 'heavy-atom' partial structure are described here. As predicted, the C(1) subunit adopts a tertiary structure typical of the lipocalin superfamily: an eight-stranded antiparallel beta-barrel with a repeated +1 topology. The beta-barrel has a calyx shape with the two molecules in the asymmetric unit interacting in such a way that the open ends of each calyx face each other, although they do not form a single elongated pocket. A comparison of this structure with those of other members of the lipocalin superfamily has allowed speculation as to the nature of carotenoid binding by the protein.
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(as it appears on PubMed at http://www.pubmed.gov), where 11526314 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11526314}}
==About this Structure==
==About this Structure==
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[[Category: Antiparallel beta barrel]]
[[Category: Antiparallel beta barrel]]
[[Category: Lipocalin fold]]
[[Category: Lipocalin fold]]
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Revision as of 07:25, 1 July 2008

Image:1i4u.png

Template:STRUCTURE 1i4u

THE C1 SUBUNIT OF ALPHA-CRUSTACYANIN

Template:ABSTRACT PUBMED 11526314

About this Structure

1I4U is a Single protein structure. Full crystallographic information is available from OCA.

Reference

The C1 subunit of alpha-crustacyanin: the de novo phasing of the crystal structure of a 40 kDa homodimeric protein using the anomalous scattering from S atoms combined with direct methods., Gordon EJ, Leonard GA, McSweeney S, Zagalsky PF, Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1230-7. Epub 2001, Aug 23. PMID:11526314

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