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| - | [[Image:1i5p.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1i5p| PDB=1i5p | SCENE= }} | | {{STRUCTURE_1i5p| PDB=1i5p | SCENE= }} |
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| - | '''INSECTICIDAL CRYSTAL PROTEIN CRY2AA'''
| + | ===INSECTICIDAL CRYSTAL PROTEIN CRY2AA=== |
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| - | ==Overview==
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| - | BACKGROUND: Genetically modified (GM) crops that express insecticidal protein toxins are an integral part of modern agriculture. Proteins produced by Bacillus thuringiensis (Bt) during sporulation mediate the pathogenicity of Bt toward a spectrum of insect larvae whose breadth depends upon the Bt strain. These transmembrane channel-forming toxins are stored in Bt as crystalline inclusions called Cry proteins. These proteins are the active agents used in the majority of biorational pesticides and insect-resistant transgenic crops. Though Bt toxins are promising as a crop protection alternative and are ecologically friendlier than synthetic organic pesticides, resistance to Bt toxins by insects is recognized as a potential limitation to their application. RESULTS: We have determined the 2.2 A crystal structure of the Cry2Aa protoxin by multiple isomorphous replacement. This is the first crystal structure of a Cry toxin specific to Diptera (mosquitoes and flies) and the first structure of a Cry toxin with high activity against larvae from two insect orders, Lepidoptera (moths and butterflies) and Diptera. Cry2Aa also provides the first structure of the proregion of a Cry toxin that is cleaved to generate the membrane-active toxin in the larval gut. CONCLUSIONS: The crystal structure of Cry2Aa reported here, together with chimeric-scanning and domain-swapping mutagenesis, defines the putative receptor binding epitope on the toxin and so may allow for alteration of specificity to combat resistance or to minimize collateral effects on nontarget species. The putative receptor binding epitope of Cry2Aa identified in this study differs from that inferred from previous structural studies of other Cry toxins.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11377201}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11377201 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11377201}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Jelly roll]] | | [[Category: Jelly roll]] |
| | [[Category: Lectin-like beta sandwich]] | | [[Category: Lectin-like beta sandwich]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:36:26 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:27:31 2008'' |
Revision as of 07:27, 1 July 2008
Template:STRUCTURE 1i5p
INSECTICIDAL CRYSTAL PROTEIN CRY2AA
Template:ABSTRACT PUBMED 11377201
About this Structure
1I5P is a Single protein structure of sequence from Bacillus thuringiensis serovar kurstaki. Full crystallographic information is available from OCA.
Reference
Structure of Cry2Aa suggests an unexpected receptor binding epitope., Morse RJ, Yamamoto T, Stroud RM, Structure. 2001 May 9;9(5):409-17. PMID:11377201
Page seeded by OCA on Tue Jul 1 10:27:31 2008
