7wmk
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wmk OCA], [https://pdbe.org/7wmk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wmk RCSB], [https://www.ebi.ac.uk/pdbsum/7wmk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wmk ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wmk OCA], [https://pdbe.org/7wmk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wmk RCSB], [https://www.ebi.ac.uk/pdbsum/7wmk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wmk ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The pyrroloquinoline quinone (PQQ)-dependent dehydrogenase DepA detoxifies deoxynivalenol (DON) by converting the C3-OH into a keto group. Herein, two crystal structures of DepA and its complex with PQQ were determined, together with biochemical evidence confirming the interactions of DepA with PQQ and DON and revealing a unique tyrosine residue important for substrate selection. Furthermore, four loops over the active site essential for DepA activity were identified, of which three loops were stabilized by PQQ, and the fourth loop invisible in both structures was considered important for binding DON, together constituting a lid for the active site. Preliminary engineering of the loop showed its potential for enzyme improvement. This study provides structural insights into how a PQQ-dependent dehydrogenase is equipped with the function of DON conversion and for the first time shows the necessity of a lid structure for PQQ-dependent dehydrogenase activity, laying foundation for structure-based design to enhance catalysis efficiency. | ||
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| + | Structure-Function Analysis of a Quinone-Dependent Dehydrogenase Capable of Deoxynivalenol Detoxification.,Yang H, Yan R, Li Y, Lu Z, Bie X, Zhao H, Lu F, Chen M J Agric Food Chem. 2022 Jun 8;70(22):6764-6774. doi: 10.1021/acs.jafc.2c01083., Epub 2022 May 25. PMID:35613468<ref>PMID:35613468</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7wmk" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 18:44, 7 September 2022
PQQ-dependent alcohol dehydrogenase complexed with PQQ
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