7qxm
From Proteopedia
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== Function == | == Function == | ||
[[https://www.uniprot.org/uniprot/A0A085R2T8_VIBCL A0A085R2T8_VIBCL]] Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.[ARBA:ARBA00003574][RuleBase:RU362085] | [[https://www.uniprot.org/uniprot/A0A085R2T8_VIBCL A0A085R2T8_VIBCL]] Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.[ARBA:ARBA00003574][RuleBase:RU362085] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | To enable chromosomal replication, DNA is unwound by the ATPase molecular motor replicative helicase. The bacterial helicase DnaB is a ring-shaped homo-hexamer whose conformational dynamics are being studied through its different 3D structural states adopted along its functional cycle. Our findings describe a new crystal structure for the apo-DnaB from Vibrio cholerae, forming a planar hexamer with pseudo-symmetry, constituted by a trimer of dimers in which the C-terminal domains delimit a triskelion-shaped hole. This hexamer is labile and inactive. We suggest that it represents an intermediate state allowing the formation of the active NTP-bound hexamer from dimers. | ||
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| + | The apo-form of the Vibrio cholerae replicative helicase DnaB is a labile and inactive planar trimer of dimers.,Cargemel C, Walbott H, Durand D, Legrand P, Ouldali M, Ferat JL, Marsin S, Quevillon-Cheruel S FEBS Lett. 2022 May 14. doi: 10.1002/1873-3468.14403. PMID:35568982<ref>PMID:35568982</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7qxm" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 18:57, 7 September 2022
Crystal structure of the Vibrio cholerae replicative helicase (DnaB)
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