4c5a
From Proteopedia
(Difference between revisions)
| Line 4: | Line 4: | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4c5a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C5A FirstGlance]. <br> | <table><tr><td colspan='2'>[[4c5a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C5A FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=DS0:[(4R)-4-AZANYL-4,5-DIHYDRO-1,2-OXAZOL-3-YL]+DIHYDROGEN+PHOSPHATE'>DS0</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=DS0:[(4R)-4-AZANYL-4,5-DIHYDRO-1,2-OXAZOL-3-YL]+DIHYDROGEN+PHOSPHATE'>DS0</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c5a OCA], [https://pdbe.org/4c5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c5a RCSB], [https://www.ebi.ac.uk/pdbsum/4c5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c5a ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c5a OCA], [https://pdbe.org/4c5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c5a RCSB], [https://www.ebi.ac.uk/pdbsum/4c5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c5a ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/DDLB_ECOLI DDLB_ECOLI] Cell wall formation. | |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | D-cycloserine is an antibiotic which targets sequential bacterial cell wall peptidoglycan biosynthesis enzymes: alanine racemase and D-alanine:D-alanine ligase. By a combination of structural, chemical and mechanistic studies here we show that the inhibition of D-alanine:D-alanine ligase by the antibiotic D-cycloserine proceeds via a distinct phosphorylated form of the drug. This mechanistic insight reveals a bimodal mechanism of action for a single antibiotic on different enzyme targets and has significance for the design of future inhibitor molecules based on this chemical structure. | ||
| + | |||
| + | Inhibition of D-Ala:D-Ala ligase through a phosphorylated form of the antibiotic D-cycloserine.,Batson S, de Chiara C, Majce V, Lloyd AJ, Gobec S, Rea D, Fulop V, Thoroughgood CW, Simmons KJ, Dowson CG, Fishwick CWG, de Carvalho LPS, Roper DI Nat Commun. 2017 Dec 5;8(1):1939. doi: 10.1038/s41467-017-02118-7. PMID:29208891<ref>PMID:29208891</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4c5a" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[D-alanine-D-alanine ligase 3D structures|D-alanine-D-alanine ligase 3D structures]] | *[[D-alanine-D-alanine ligase 3D structures|D-alanine-D-alanine ligase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
The X-ray crystal structures of D-alanyl-D-alanine ligase in complex ADP and D-cycloserine phosphate
| |||||||||||
