7v9x

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7v9x]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V9X FirstGlance]. <br>
<table><tr><td colspan='2'>[[7v9x]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V9X FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v9x OCA], [https://pdbe.org/7v9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v9x RCSB], [https://www.ebi.ac.uk/pdbsum/7v9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v9x ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.82&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v9x OCA], [https://pdbe.org/7v9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v9x RCSB], [https://www.ebi.ac.uk/pdbsum/7v9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v9x ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/RT86_ECOLX RT86_ECOLX]] Reverse transcriptase (RT) component of antiviral defense system retron Ec86, composed of a non-coding RNA (ncRNA), a ribosyltransferase/DNA-binding protein and this RT. Expression of the 3-gene retron confers protection against bacteriophage T5. At multiplicity of infection (MOI) of 0.02 cultures grow normally when infected with T5 without collapsing, at MOI 2 cultures enter growth stasis (PubMed:33157039). Responsible for synthesis of msDNA (a branched molecule with RNA linked by a 2',5'-phosphodiester bond to ssDNA). The retron transcript serves as primer (from a conserved internal G residue) and template for the reaction, and codes for the RT (PubMed:2466573, PubMed:10531319). Recognizes only its cognate RNA as a primer template (PubMed:10531319). Overexpression of the ncRNA and RT (without the ribosyltransferase), which leads to increased levels of msDNA, is mutagenic in vivo (PubMed:7885227). This may be due to a mismatch in the msDNA stem which binds and sequesters MutS and/or MutL (Probable).<ref>PMID:10531319</ref> <ref>PMID:2466573</ref> <ref>PMID:33157039</ref> <ref>PMID:7885227</ref> <ref>PMID:7885227</ref>
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[https://www.uniprot.org/uniprot/RT86_ECOLX RT86_ECOLX] Reverse transcriptase (RT) component of antiviral defense system retron Ec86, composed of a non-coding RNA (ncRNA), a ribosyltransferase/DNA-binding protein and this RT. Expression of the 3-gene retron confers protection against bacteriophage T5. At multiplicity of infection (MOI) of 0.02 cultures grow normally when infected with T5 without collapsing, at MOI 2 cultures enter growth stasis (PubMed:33157039). Responsible for synthesis of msDNA (a branched molecule with RNA linked by a 2',5'-phosphodiester bond to ssDNA). The retron transcript serves as primer (from a conserved internal G residue) and template for the reaction, and codes for the RT (PubMed:10531319, PubMed:2466573). Recognizes only its cognate RNA as a primer template (PubMed:10531319). Overexpression of the ncRNA and RT (without the ribosyltransferase), which leads to increased levels of msDNA, is mutagenic in vivo (PubMed:7885227). This may be due to a mismatch in the msDNA stem which binds and sequesters MutS and/or MutL (Probable).<ref>PMID:10531319</ref> <ref>PMID:2466573</ref> <ref>PMID:33157039</ref> <ref>PMID:7885227</ref> <ref>PMID:7885227</ref>
== References ==
== References ==
<references/>
<references/>

Current revision

Cryo-EM structure of E.coli retron-Ec86 in complex with its effector at 2.8 angstrom

PDB ID 7v9x

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