7qx7

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qx7 OCA], [https://pdbe.org/7qx7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qx7 RCSB], [https://www.ebi.ac.uk/pdbsum/7qx7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qx7 ProSAT]</span></td></tr>

[[https://www.uniprot.org/uniprot/C11AA_BACTI C11AA_BACTI]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of mosquitos.

Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control.

 

== References ==