7f23
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[7f23]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F23 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7f23]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F23 FirstGlance]. <br> | ||
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=LDP:L-DOPAMINE'>LDP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.58Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=LDP:L-DOPAMINE'>LDP</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f23 OCA], [https://pdbe.org/7f23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f23 RCSB], [https://www.ebi.ac.uk/pdbsum/7f23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f23 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f23 OCA], [https://pdbe.org/7f23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f23 RCSB], [https://www.ebi.ac.uk/pdbsum/7f23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f23 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | == | + | <div style="background-color:#fffaf0;"> |
| - | + | == Publication Abstract from PubMed == | |
| + | G protein-coupled receptors (GPCRs) comprise the largest family of membrane receptors and are the most important drug targets. An agonist-bound GPCR engages heterotrimeric G proteins and triggers the exchange of guanosine diphosphate (GDP) with guanosine triphosphate (GTP) to promote G protein activation. A complete understanding of molecular mechanisms of G protein activation has been hindered by a lack of structural information of GPCR-G protein complex in nucleotide-bound states. Here, we report the cryo-EM structures of the D1 dopamine receptor and mini-G(s) complex in the nucleotide-free and nucleotide-bound states. These structures reveal major conformational changes in Galpha such as structural rearrangements of the carboxyl- and amino-terminal alpha helices that account for the release of GDP and the GTP-dependent dissociation of Galpha from Gbetagamma subunits. As validated by biochemical and cellular signaling studies, our structures shed light into the molecular basis of the entire signaling events of GPCR-mediated G protein activation. | ||
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| + | Structural insights into G protein activation by D1 dopamine receptor.,Teng X, Chen S, Wang Q, Chen Z, Wang X, Huang N, Zheng S Sci Adv. 2022 Jun 10;8(23):eabo4158. doi: 10.1126/sciadv.abo4158. Epub 2022 Jun , 10. PMID:35687690<ref>PMID:35687690</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7f23" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Dopamine receptor 3D structures|Dopamine receptor 3D structures]] | ||
| + | *[[Transducin 3D structures|Transducin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Cryo-EM structure of the GTP-bound dopamine receptor 1 and mini-Gs complex with Nb35
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