8guo

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "8guo" [edit=sysop:move=sysop])
Line 1: Line 1:
-
'''Unreleased structure'''
 
-
The entry 8guo is ON HOLD
+
==Crystal structure of the nuclease domain of EsaD in complex with EsaG from Staphylococcus aureus==
 +
<StructureSection load='8guo' size='340' side='right'caption='[[8guo]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[8guo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GUO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GUO FirstGlance]. <br>
 +
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8guo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8guo OCA], [https://pdbe.org/8guo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8guo RCSB], [https://www.ebi.ac.uk/pdbsum/8guo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8guo ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ESSG_STAA8 ESSG_STAA8] Component of the type VII secretion system (Ess). Acts also as part of toxin-antitoxin system. Counteracts the toxic effect of EssD via direct interaction.<ref>PMID:27723728</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Toxin EsaD secreted by some S. aureus strains through the type VII secretion system (T7SS) specifically kills those strains lacking the antitoxin EsaG. Here we report the structures of EsaG, the nuclease domain of EsaD and their complex, which together reveal an inhibition mechanism that relies on significant conformational change of the toxin. To inhibit EsaD, EsaG breaks the nuclease domain of EsaD protein into two independent fragments that, in turn, sandwich EsaG. The originally well-folded betabetaalpha-metal finger connecting the two fragments is stretched to become a disordered loop, leading to disruption of the catalytic site of EsaD and loss of nuclease activity. This mechanism is distinct from that of the other Type II toxin-antitoxin systems, which utilize an intrinsically disordered region on the antitoxins to cover the active site of the toxins. This study paves the way for developing therapeutic approaches targeting this antagonism.
-
Authors: Zhang, Z.M., Wang, Y.J.
+
A toxin-deformation dependent inhibition mechanism in the T7SS toxin-antitoxin system of Gram-positive bacteria.,Wang Y, Zhou Y, Shi C, Liu J, Lv G, Huang H, Li S, Duan L, Zheng X, Liu Y, Zhou H, Wang Y, Li Z, Ding K, Sun P, Huang Y, Lu X, Zhang ZM Nat Commun. 2022 Oct 28;13(1):6434. doi: 10.1038/s41467-022-34034-w. PMID:36307446<ref>PMID:36307446</ref>
-
Description: Crystal structure of the nuclease domain of EsaD in complex with EsaG from Staphylococcus aureus
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Unreleased Structures]]
+
</div>
-
[[Category: Wang, Y.J]]
+
<div class="pdbe-citations 8guo" style="background-color:#fffaf0;"></div>
-
[[Category: Zhang, Z.M]]
+
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
 +
[[Category: Wang YJ]]
 +
[[Category: Zhang ZM]]

Revision as of 07:38, 9 November 2022

Crystal structure of the nuclease domain of EsaD in complex with EsaG from Staphylococcus aureus

PDB ID 8guo

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/8guo"
Personal tools