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1ik7

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{{STRUCTURE_1ik7| PDB=1ik7 | SCENE= }}
{{STRUCTURE_1ik7| PDB=1ik7 | SCENE= }}
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'''Crystal Structure of the Uncomplexed Pelle Death Domain'''
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===Crystal Structure of the Uncomplexed Pelle Death Domain===
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==Overview==
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The death domain (DD) of the protein kinase Pelle adopts a six-helix bundle fold in the crystal structure of the complex with its dimerization partner, Tube-DD. However, in crystals obtained from a solution of 45% 2-methyl-2,4-pentanediol (MPD), the C-terminal half of Pelle-DD folds into a single helix, and the N-terminal half of the molecule is disordered. The helical segment forms an antiparallel dimer with the corresponding helix of a symmetry-related molecule, and together they form extensive lattice interactions similar in number, composition, and buried surface to those in the six-helix bundle of the native fold. Secondary structure analysis by heteronuclear nuclear magnetic resonance spectroscopy (NMR) demonstrates that Pelle-DD adopts a six-helix bundle fold in aqueous solution. The fold is perturbed by MPD, with the largest chemical shift changes in one helix and two loop regions that encompass the Tube-DD binding site. Pelle-DD is stable to urea denaturation with a folding free energy of 7.9 kcal/mol at 25 degrees C but is destabilized, with loss of urea binding sites, in the presence of MPD. The data are consistent with a cosolvent denaturation model in which MPD denatures the N terminus of Pelle-DD but induces the C terminus to form a more compact structure and aggregate. A similar perturbation in vivo might occur at the plasma membrane and could have consequences for Pelle-mediated regulation. Generally, crystallographers should be aware that high concentrations of MPD or related cosolvents can alter the tertiary structure of susceptible proteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 12177432 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12177432}}
==About this Structure==
==About this Structure==
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[[Category: Single helix]]
[[Category: Single helix]]
[[Category: Structural transition]]
[[Category: Structural transition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:21:31 2008''

Revision as of 10:21, 1 July 2008

Image:1ik7.png

Template:STRUCTURE 1ik7

Crystal Structure of the Uncomplexed Pelle Death Domain

Template:ABSTRACT PUBMED 12177432

About this Structure

1IK7 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

Reference

Cosolvent-induced transformation of a death domain tertiary structure., Xiao T, Gardner KH, Sprang SR, Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11151-6. Epub 2002 Aug 12. PMID:12177432

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