2iwf
From Proteopedia
(New page: 200px<br /> <applet load="2iwf" size="450" color="white" frame="true" align="right" spinBox="true" caption="2iwf, resolution 1.86Å" /> '''RESTING FORM OF PIN...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2IWF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Achromobacter_cycloclastes Achromobacter cycloclastes]] with CU, CA, CL, NA and S as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.6 1.7.99.6]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IWF OCA]]. | + | 2IWF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Achromobacter_cycloclastes Achromobacter cycloclastes]] with CU, CA, CL, NA and S as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Nitrous-oxide_reductase Nitrous-oxide reductase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.6 1.7.99.6]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IWF OCA]]. |
==Reference== | ==Reference== | ||
Insight into catalysis of nitrous oxide reductase from high-resolution structures of resting and inhibitor-bound enzyme from Achromobacter cycloclastes., Paraskevopoulos K, Antonyuk SV, Sawers RG, Eady RR, Hasnain SS, J Mol Biol. 2006 Sep 8;362(1):55-65. Epub 2006 Jul 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16904686 16904686] | Insight into catalysis of nitrous oxide reductase from high-resolution structures of resting and inhibitor-bound enzyme from Achromobacter cycloclastes., Paraskevopoulos K, Antonyuk SV, Sawers RG, Eady RR, Hasnain SS, J Mol Biol. 2006 Sep 8;362(1):55-65. Epub 2006 Jul 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16904686 16904686] | ||
[[Category: Achromobacter cycloclastes]] | [[Category: Achromobacter cycloclastes]] | ||
| + | [[Category: Nitrous-oxide reductase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Antonyuk, S.V.]] | [[Category: Antonyuk, S.V.]] | ||
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[[Category: periplasmic electron gating]] | [[Category: periplasmic electron gating]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:38:19 2007'' |
Revision as of 09:33, 30 October 2007
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RESTING FORM OF PINK NITROUS OXIDE REDUCTASE FROM ACHROMOBACTER CYCLOCLASTES
Overview
The difficult chemistry of nitrous oxide (N2O) reduction to gaseous, nitrogen (N2) in biology is catalysed by the novel micro4-sulphide-bridged, tetranuclear Cuz cluster of the N2O reductases (N2OR). Two, spectroscopically distinct forms of this cluster have been identified as, CuZ and CuZ*. We have obtained a 1.86 A resolution crystal structure of, the pink-purple species of N2OR from Achromobacter cycloclastes (AcN2OR), isolated under aerobic conditions. This structure reveals a previously, unobserved ligation with two oxygen atoms from H2O/OH- coordinated to Cu1, and Cu4 of the catalytic centre. We ascribe this structure to be that of, the CuZ form of the cluster, since the previously reported structures of, two blue species of N2ORs, also isolated aerobically, have characterised, the ... [(full description)]
About this Structure
2IWF is a [Single protein] structure of sequence from [Achromobacter cycloclastes] with CU, CA, CL, NA and S as [ligands]. Active as [Nitrous-oxide reductase], with EC number [1.7.99.6]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Insight into catalysis of nitrous oxide reductase from high-resolution structures of resting and inhibitor-bound enzyme from Achromobacter cycloclastes., Paraskevopoulos K, Antonyuk SV, Sawers RG, Eady RR, Hasnain SS, J Mol Biol. 2006 Sep 8;362(1):55-65. Epub 2006 Jul 12. PMID:16904686
Page seeded by OCA on Tue Oct 30 11:38:19 2007
Categories: Achromobacter cycloclastes | Nitrous-oxide reductase | Single protein | Antonyuk, S.V. | Eady, R.R. | Hasnain, S.S. | Paraskevopoulos, K. | Sawers, R.G. | CA | CL | CU | NA | S | Calcium | Catalysis | Copper | Copper chemistry | Denitrification | Metal-binding | Nitrous oxide binding | Oxidoreductase | Periplasmic electron gating
