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1ikl

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{{STRUCTURE_1ikl| PDB=1ikl | SCENE= }}
{{STRUCTURE_1ikl| PDB=1ikl | SCENE= }}
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'''NMR STUDY OF MONOMERIC HUMAN INTERLEUKIN-8 (MINIMIZED AVERAGE STRUCTURE)'''
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===NMR STUDY OF MONOMERIC HUMAN INTERLEUKIN-8 (MINIMIZED AVERAGE STRUCTURE)===
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==Overview==
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The solution structure of a monomeric form of interleukin-8 (IL-8) has been solved using 1H NMR spectroscopy. The chemically synthesized nonnatural analog [IL-8 (4-72) L25 NH--&gt;NCH3] has the same activity as that of native IL-8. Thirty structures were generated using the hybrid distance geometry and simulated annealing protocol using the program X-PLOR. The structure is well-defined except for N-terminal residues 4-6 and C-terminal residues 67-72. The rms distribution about the average structure for residues 7-66 is 0.38 A for the backbone atoms and 0.87 A for all heavy atoms. The structure consists of a series of turns and loops followed by a triple-stranded beta sheet and a C-terminal alpha helix. The structure of the monomer is largely similar to the native dimeric IL-8 structures previously determined by both NMR and X-ray methods. The major difference is that, in the monomeric analog, the C-terminal residues 67-72 are disordered whereas they are helical in the two dimeric structures. The best fit superposition of the backbone atoms of residues 7-66 of the monomer structure on the dimeric IL-8 structures showed rms differences of 1.5 and 1.2 A respectively. The turn (residues 31-35), which is disulfide linked to the N-terminal region, adopts a conformation in the monomer similar to that seen in the dimeric X-ray structure (rms difference 1.4 A) and different from that seen in the dimeric NMR structure (rms difference 2.7 A).(ABSTRACT TRUNCATED AT 250 WORDS)
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{{ABSTRACT_PUBMED_7548056}}
==About this Structure==
==About this Structure==
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1IKL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IKL OCA].
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1IKL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IKL OCA].
==Reference==
==Reference==
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[[Category: Rajarathnam, K.]]
[[Category: Rajarathnam, K.]]
[[Category: Sykes, B D.]]
[[Category: Sykes, B D.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:06:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:23:43 2008''

Revision as of 10:23, 1 July 2008

Image:1ikl.png

Template:STRUCTURE 1ikl

NMR STUDY OF MONOMERIC HUMAN INTERLEUKIN-8 (MINIMIZED AVERAGE STRUCTURE)

Template:ABSTRACT PUBMED 7548056

About this Structure

1IKL is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

1H NMR solution structure of an active monomeric interleukin-8., Rajarathnam K, Clark-Lewis I, Sykes BD, Biochemistry. 1995 Oct 10;34(40):12983-90. PMID:7548056

Page seeded by OCA on Tue Jul 1 13:23:43 2008

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