1in6

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1in6.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1in6.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1in6| PDB=1in6 | SCENE= }}
{{STRUCTURE_1in6| PDB=1in6 | SCENE= }}
-
'''THERMOTOGA MARITIMA RUVB K64R MUTANT'''
+
===THERMOTOGA MARITIMA RUVB K64R MUTANT===
-
==Overview==
+
<!--
-
The RuvB hexamer is the chemomechanical motor of the RuvAB complex that migrates Holliday junction branch-points in DNA recombination and the rescue of stalled DNA replication forks. The 1.6 A crystal structure of Thermotoga maritima RuvB together with five mutant structures reveal that RuvB is an ATPase-associated with diverse cellular activities (AAA+-class ATPase) with a winged-helix DNA-binding domain. The RuvB-ADP complex structure and mutagenesis suggest how AAA+-class ATPases couple nucleotide binding and hydrolysis to interdomain conformational changes and asymmetry within the RuvB hexamer implied by the crystallographic packing and small-angle X-ray scattering in solution. ATP-driven domain motion is positioned to move double-stranded DNA through the hexamer and drive conformational changes between subunits by altering the complementary hydrophilic protein- protein interfaces. Structural and biochemical analysis of five motifs in the protein suggest that ATP binding is a strained conformation recognized both by sensors and the Walker motifs and that intersubunit activation occurs by an arginine finger motif reminiscent of the GTPase-activating proteins. Taken together, these results provide insights into how RuvB functions as a motor for branch migration of Holliday junctions.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11478862}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11478862 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11478862}}
==About this Structure==
==About this Structure==
Line 36: Line 40:
[[Category: Walker b]]
[[Category: Walker b]]
[[Category: Winged-helix domain]]
[[Category: Winged-helix domain]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:10:43 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:35:25 2008''

Revision as of 10:35, 1 July 2008

Image:1in6.png

Template:STRUCTURE 1in6

THERMOTOGA MARITIMA RUVB K64R MUTANT

Template:ABSTRACT PUBMED 11478862

About this Structure

1IN6 is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of the RuvB Holliday junction branch migration motor., Putnam CD, Clancy SB, Tsuruta H, Gonzalez S, Wetmur JG, Tainer JA, J Mol Biol. 2001 Aug 10;311(2):297-310. PMID:11478862

Page seeded by OCA on Tue Jul 1 13:35:25 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1in6"
Personal tools